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Table 2 Relative fold-changes of mdtA and mdtU mRNA transcripts in E. amylovora Ea1189 after 2 h of incubation with transporter substrates as determined by qRT-PCR a

From: Characterization and regulation of the Resistance-Nodulation-Cell Division-type multidrug efflux pumps MdtABC and MdtUVW from the fire blight pathogen Erwinia amylovora

  mdtA b mdtU
Methanolic apple extract (1 μl/ml) 1.2 1.2
Acetonic apple extract (10 μl/ml) 1.2 1.5
Tannin (0.5 mg/ml) 4.4 1.7
Phloretin (4 μg/ml) 0.8 0.9
Naringenin (8 μg/ml) 1.1 1.1
Myricetin (10 μg/ml) 0.8 0.7
Indole (2 mM) 1.0 0.7
Paraquat (0.2 mM) 0.9 1.3
Phenolic acidsc (0.078 mM) 0.9 0.7
Gallic acid (1 mg/ml) 1.4 1.5
Indole-3-acetic acid (2 mM) 0.8 0.7
Iron sulphate (1 mM) 0.9 0.8
Copper sulphate (1 mM) 1.1 1.4
Zinc sulphate (1 mM) 1.1 1.4
Sodium Tungstate (20 mM) 2.4 1.2
  1. aTotal RNA was isolated from bacterial cells incubated for 2 hours with transporter substrates in LB broth. Transcript abundance was determined by quantitative RT-PCR and compared to RT-PCR signal from cells grown in LB broth containing only the solvent of the respective substrate.
  2. bBoldface values indicate an increase of at least 2-fold. Represented data values are the means of at least three replicates.
  3. cA mixture of 0.078 mM salicylic acid and 0.078 mM benzoic acid was used.