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Table 1 Antimicrobial susceptibility profiles

From: Characterization and regulation of the Resistance-Nodulation-Cell Division-type multidrug efflux pumps MdtABC and MdtUVW from the fire blight pathogen Erwinia amylovora

Drug MIC (μg/ml)a
  Ea1189 Ea1189.ΔmdtABC Ea1189.ΔmdtUVW Ea1189-3 Ea1189-3 pBlueSK.mdtABC Ea1189-3 pBlueKS.mdtUVW
Phytochemicals       
Apple extract > 100000 > 100000 > 100000 25000 50000 25000
Tannin 625 625 625 1250 5000 1250
Flavones       
Apigenin > 2500 > 2500 > 2500 31.2 125 > 1000
Daidzein 1000 1000 1000 156 1000 1000
Genistein > 1000 > 1000 > 1000 62.5 125 > 1000
Kaempferol > 5000 > 5000 > 5000 250 1000 1000
Luteolin > 1000 > 1000 > 1000 12.5 25 > 100
Myricetin 2500 2500 2500 1250 1250 1250
Naringenin 1000 1000 1000 250 250 1000
Quercitin 5000 5000 5000 2500 2500 5000
Orobol 1000 1000 1000 31.2 31.2 1000
β-Lactams       
Ampicillin 312 312 312 25 50 50
Carbenicillin 312 312 312 50 50 50
Cefepime 12.5 12.5 12.5 0.16 0.31 0.16
Cefoxitin 62.5 62.5 62.5 25 25 25
Cefsulodin 156 156 156 50 50 50
Ceftazidime 1000 1000 1000 6.2 6.2 6.2
Cloxacillin 2500 2500 2500 25 25 25
Oxacillin 1250 1250 1250 12.5 25 25
Ticarcillin 250 250 250 50 50 50
Aminoglycosides       
Amikacin 3.1 3.1 3.1 5 5 5
Gentamicin 2.5 2.5 2.5 2.5 2.5 2.5
Kanamycin 6.2 6.2 6.2 > 100 > 100 > 100
Neomycin 3.1 3.1 3.1 > 100 > 100 > 100
Streptomycin 3.1 3.1 3.1 5 5 5
Tobramycin 1.2 1.2 1.2 5 5 5
Antibiotics       
Chloramphenicol 3.1 3.1 3.1 1.5 1.5 1.5
Erythromycin 1.2 1.2 1.2 0.3 0.3 0.6
Fosfomycin 1250 1250 1250 156 312 156
Fusidic acid 250 250 250 6.2 62.5 100
Josamycin 250 250 250 6.2 50 12.5
Lincomycin 1250 1250 1250 78 78 78
Norfloxacin 0.13 0.13 0.13 0.03 0.03 0.06
Novobiocin 125 125 125 6.2 100 100
Rifampicin 50 50 50 31.2 31.2 50
Tetracycline 2.5 2.5 2.5 0.63 1.25 1.25
Trimethoprim 625 625 625 625 625 625
Dyes       
Acriflavine 31.2 31.2 31.2 25 25 25
Ethidium bromide 500 500 500 6.2 6.2 6.2
Antimicrobials       
Bile 5000 5000 5000 625 2500 1250
Clotrimazole > 1000 > 1000 > 1000 6.2 6.2 > 100
Indole 625 625 625 625 625 625
SDS 625 625 625 125 125 250
Heavy metals       
Cadmium acetate 25 25 25 25 25 25
Copper sulfate 1250 1250 1250 1250 1250 1250
Silver nitrate 6.2 6.2 6.2 6.2 25 6.25
Sodium tungstate 125000 125000 125000 62500 62500 62500
Zinc sulfate 312 312 312 312 625 312
  1. aMIC values were determined by the 2-fold dilution assay in three or more independent experiments with similar results. Boldface numbers indicate a more than 2-fold higher MIC. ND, not determined.
  2. MIC values were determined from an E. amylovora wild-type strain, mdtABC mutant, and mdtUVW mutant, as well as from an acrB mutant Ea1189-3 complemented with MdtABC-overexpression plasmid pBlueSK.mdtABC or MdtUVW-overexpression plasmid pBlueKS.mdtUVW-ext.