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Figure 2 | BMC Microbiology

Figure 2

From: Characterization and regulation of the Resistance-Nodulation-Cell Division-type multidrug efflux pumps MdtABC and MdtUVW from the fire blight pathogen Erwinia amylovora

Figure 2

Electrophoretic mobility shift analysis of BaeR and CpxR interaction with Cy5-labeled DNA fragments. DNA fragments contain the promoter regions of (A) mdtABC (290 bp), (B) mdtUVW (276 bp) and (C) a fragment from within the tolC gene (248 bp) as control. Approximately 0.3 pmol of the DNA fragments were incubated with increasing amounts of His-tag purified BaeR and CpxR, respectively (indicated at the top of the panel). The DNA-protein complexes were separated on 4% non-denaturing polyacrylamide gels.

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BMC Microbiology

ISSN: 1471-2180

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