Table 2 Primers Used in This Study
Primers* | Nucleotide Sequence (5′ - > 3′) | Purpose |
|---|---|---|
ComX-F1 | CGGGATCC ATGGAAGAAGATTTTGAAATTG | GST fusion |
ComX-Not1-B | AAGCGGCCGC AGGTACAATCACATGTTCCATTC | |
MecA-F1 | CGGGATCC ATGGAAATGAAACAAATCAGC | GST fusion |
MecA-Not1-B | AAGCGGCCGC AGGTATCATCTAGCTTATCCAATC | |
ClpC-F1 | CGGGATCC CCATCTATGACCGATTACTCA | GST fusion |
ClpC-Not1-B | AAGCGGCCGC AGGACACTGAAGTAGGGTAAAG | |
ClpP-F1 | CGGGATCC ATGATTCCTGTAGTTATTGA | GST fusion |
ClpP-Not1-B | AAGCGGCCGC ACATGGTTTTTCTACTCAAC | |
ComX-BamH1-F | CGGGATCC CATGGAAGAAGATTTTGAAATTG | His fusion |
ComX-B3 | AAGCGGCCGC TTTTTCCTTAAAATCACTTAATTT | |
MecA-BamH1-F | CGGGATCC CATGGAAATGAAACAAATCAGC | His fusion |
MecA-B3 | AAGCGGCCGC TCCAATCATTTGTAATTCTTGC | |
ClpC-BamH1-F | CGGGATCC CCCATCTATGACCGATTACTCA | His fusion |
ClpC-B3 | AAGCGGCCGC TACAATATCAAATTTTAATTTTTC | |
ClpP-BamH1-F | CGGGATCC CATGATTCCTGTAGTTATTGA | His fusion |
ClpP-B3 | AAGCGGCCGC TTTTAATTCATTATTTTCCA | |
NTD-BamH1-F | CGGGATCC GATGGAAATGAAACAAATCAGC | NTD-His fusion |
NTD-Not1-B | AAGCGGCCGC TTTATTGATTTCTGACTTAGTGACA | |
CTD-BamH1-F | CGGGATCC GTTGGCTGAAATTGAAAAAAGAGA | CTD-His fusion |
CTD-Not1-B | AAGCGGCCGC TCCAATCATTTGTAATTCTTGCAG | |
MecA-EcoR1-F1 | CGGAATTC CACTTTCTTTCTCGGAATACC | MecA-His |
MecA-Not1-B1 | AAGCGGCCGC TCCAATCATTTGTAATTCTTGC | |
MecA-BamH1-B2 | CGGGATCC CAAAAAACCCCTCAAGACC | |
ClpC-BamH1-F | CGGGATCC TAGCGAGCAAGTTTTTACA | ClpC-His |
ClpC-Not1-B1 | AAGCGGCCGC TATGAAAAATAGACTAGATAAGTAT | |
ClpC-Not1-B2 | AAGCGGCCGC TACAATATCAAATTTTAATTTTTC | |
ClpP-EcoR1-F | CGGAATTC CGAGCTTAAAATGGGATAAC | ClpP-His |
ClpP-Not1-B1 | AAGCGGCCGC GTATTTTAATTCATTATTTTCCAT | |
ClpP-Not1-B2 | AAGCGGCCGC TTTTAATTCATTATTTTCCATGA | |
Pldh-Sac1-F | CGAGCTC CCGAGCAACAATAACACTC | Pldh cloning |
Pldh-Spe1-B | GGACTAGT AACATCTCCTTATAATTTATTAAGTATATATTCTAT | |
MecA-Ox-F | GGACTAGT ATGGAAATGAAACAAATCAGC | MecA over expression |
MecA-Ox-B | CGGGATCC CAAAAAACCCCTCAAGACC |