|
ComX-F1
|
CGGGATCC ATGGAAGAAGATTTTGAAATTG
|
GST fusion
|
|
ComX-Not1-B
|
AAGCGGCCGC AGGTACAATCACATGTTCCATTC
|
|
MecA-F1
|
CGGGATCC ATGGAAATGAAACAAATCAGC
|
GST fusion
|
|
MecA-Not1-B
|
AAGCGGCCGC AGGTATCATCTAGCTTATCCAATC
|
|
ClpC-F1
|
CGGGATCC CCATCTATGACCGATTACTCA
|
GST fusion
|
|
ClpC-Not1-B
|
AAGCGGCCGC AGGACACTGAAGTAGGGTAAAG
|
|
ClpP-F1
|
CGGGATCC ATGATTCCTGTAGTTATTGA
|
GST fusion
|
|
ClpP-Not1-B
|
AAGCGGCCGC ACATGGTTTTTCTACTCAAC
|
|
ComX-BamH1-F
|
CGGGATCC CATGGAAGAAGATTTTGAAATTG
|
His fusion
|
|
ComX-B3
|
AAGCGGCCGC TTTTTCCTTAAAATCACTTAATTT
|
|
MecA-BamH1-F
|
CGGGATCC CATGGAAATGAAACAAATCAGC
|
His fusion
|
|
MecA-B3
|
AAGCGGCCGC TCCAATCATTTGTAATTCTTGC
|
|
ClpC-BamH1-F
|
CGGGATCC CCCATCTATGACCGATTACTCA
|
His fusion
|
|
ClpC-B3
|
AAGCGGCCGC TACAATATCAAATTTTAATTTTTC
|
|
ClpP-BamH1-F
|
CGGGATCC CATGATTCCTGTAGTTATTGA
|
His fusion
|
|
ClpP-B3
|
AAGCGGCCGC TTTTAATTCATTATTTTCCA
|
|
NTD-BamH1-F
|
CGGGATCC GATGGAAATGAAACAAATCAGC
|
NTD-His fusion
|
|
NTD-Not1-B
|
AAGCGGCCGC TTTATTGATTTCTGACTTAGTGACA
|
|
CTD-BamH1-F
|
CGGGATCC GTTGGCTGAAATTGAAAAAAGAGA
|
CTD-His fusion
|
|
CTD-Not1-B
|
AAGCGGCCGC TCCAATCATTTGTAATTCTTGCAG
|
|
MecA-EcoR1-F1
|
CGGAATTC CACTTTCTTTCTCGGAATACC
|
MecA-His
|
|
MecA-Not1-B1
|
AAGCGGCCGC TCCAATCATTTGTAATTCTTGC
|
|
MecA-BamH1-B2
|
CGGGATCC CAAAAAACCCCTCAAGACC
|
|
ClpC-BamH1-F
|
CGGGATCC TAGCGAGCAAGTTTTTACA
|
ClpC-His
|
|
ClpC-Not1-B1
|
AAGCGGCCGC TATGAAAAATAGACTAGATAAGTAT
|
|
ClpC-Not1-B2
|
AAGCGGCCGC TACAATATCAAATTTTAATTTTTC
|
|
ClpP-EcoR1-F
|
CGGAATTC CGAGCTTAAAATGGGATAAC
|
ClpP-His
|
|
ClpP-Not1-B1
|
AAGCGGCCGC GTATTTTAATTCATTATTTTCCAT
|
|
ClpP-Not1-B2
|
AAGCGGCCGC TTTTAATTCATTATTTTCCATGA
|
|
Pldh-Sac1-F
|
CGAGCTC CCGAGCAACAATAACACTC
|
Pldh cloning
|
|
Pldh-Spe1-B
|
GGACTAGT AACATCTCCTTATAATTTATTAAGTATATATTCTAT
|
|
MecA-Ox-F
|
GGACTAGT ATGGAAATGAAACAAATCAGC
|
MecA over expression
|
|
MecA-Ox-B
|
CGGGATCC CAAAAAACCCCTCAAGACC
|
