|
S. mutans
|
|
UA159
|
Wild type, the genome sequence reference strain
|
48
|
|
XT-D1
|
UA159 ∆comX::spec, Specr
|
34
|
|
XT-D3
|
UA159 ∆mecA::erm, Ermr
|
34
|
|
XT-D4
|
UA159 ∆mecA::spec, Specr
|
34
|
|
XT-D7
|
UA159 ∆clpC::kan, Kanr
|
This study
|
|
XT-D8
|
UA159 ∆clpP::kan, Kanr
|
This study
|
|
XT-Lx1
|
UA159 carrying pWAR304, Emr
,
|
34
|
|
XT-His1
|
UA159 carrying pSigX-His, Specr
|
34
|
|
XT-His2
|
XT-D3 carrying pSigX-His, Emr
, Specr
|
34
|
|
XT-His3
|
XT-D7 carrying pSigX-His, Kanr
, Specr
|
34
|
|
GF-His1
|
XT-D8 carrying pSigX-His, Kanr
, Specr
|
This study
|
|
GF-His2
|
UA159 carrying pMecA-His, Specr
|
This study
|
|
GF-His3
|
UA159 carrying pClpC-His, Specr
|
This study
|
|
GF-His4
|
UA159 carrying pClpP-His, Specr
|
This study
|
|
GF-Ox
|
UA159 pDL277::Pldh-mecA-His, Specr
|
This study
|
|
E. coli
|
|
DH5α
|
Cloning host
|
Invitrogen
|
|
XL1-blue
|
Cloning host
|
Stratagene
|
|
BL21(DE3)
|
Recombinant protein expression strain
|
Novagen
|
|
BL21(DE3)pLysS
|
Recombinant protein expression strain
|
Novagen
|
|
Plasmids
|
|
pGEX-6P-1
|
E. coli expression vector (GST-tag), Ampr
|
GE
|
|
pGF-SigX
|
pGEX-6P-1::comX, GST-tag fusion, Ampr
|
This study
|
|
pGF-MecA
|
pGEX-6P-1::mecA, GST-tag fusion, Ampr
|
This study
|
|
pGF-ClpC
|
pGEX-6P-1::clpC, GST-tag fusion, Ampr
|
This study
|
|
pGF-ClpP
|
pGEX-6P-1::clpP, GST-tag fusion, Ampr
|
This study
|
|
pET-20b(+)
|
E. coli expression vector (6His-tag), Ampr
|
Novagen
|
|
pGF2-SigX
|
pET-20b::comX, His-tag fusion, Ampr
|
This study
|
|
pGF2-MecA
|
pET-20b::mecA; His-tag fusion, Ampr;
|
This study
|
|
pGF2-ClpC
|
pET-20b::clpC; His-tag fusion, Ampr
|
This study
|
|
pGF2-ClpP
|
pET-20b::clpP; His-tag fusion, Ampr
|
This study
|
|
pGF2-NTD
|
pET-20b::mecA
(aa1–82)
, His-tag fusion, Ampr;
|
This study
|
|
pGF2-CTD
|
pET-20b::mecA
(aa123–240)
, His-tag fusion, Ampr;
|
This study
|
|
pGF3-P + X
|
pET-20b::Promoter + comX, His-tag fusion, Ampr
|
This study
|
|
pDL277
|
E. coli-Streptococcus shuttle vector, Specr
|
47
|
|
pSigX-His
|
pDL277::Promoter + comX-His, Specr
|
34
|
|
pGF3-PA
|
pET-20b::Promoter + mecA, His-tag fusion, Ampr
|
This study
|
|
pMecA-His
|
pDL277::Promoter + mecA-His, Specr
|
This study
|
|
pGF3-PC
|
pET-20b::Promoter + clpC, His-tag fusion, Ampr
|
This study
|
|
pClpC-His
|
pDL277::Promoter + clpC-His, Specr
|
This study
|
|
pGF3-PP
|
pET-20b::Promoter + clpP, His-tag fusion, Ampr
|
This study
|
|
pClpP-His
|
pDL277::Promoter + clpP-His, Specr
|
This study
|
|
pXT-Pldh
|
pDL277::Promoter of ldh (Pldh), Specr
|
This study
|
|
pMecA-Ox
|
pXT-Pldh::MecA-His, Specr
|
This study
|
|
pWAR303
|
A shuttle vector containing promoterless luxAB, Emr
|
18
|
|
pWAR304
|
pWAR303 having a fusion of PcomX::luxAB, Emr
|
18
|
