Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Regulated proteolysis of the alternative sigma factor SigX in Streptococcus mutans: implication in the escape from competence

Strains Relevant characteristics Source or reference
S. mutans
UA159 Wild type, the genome sequence reference strain 48
XT-D1 UA159 ∆comX::spec, Specr 34
XT-D3 UA159 ∆mecA::erm, Ermr 34
XT-D4 UA159 ∆mecA::spec, Specr 34
XT-D7 UA159 ∆clpC::kan, Kanr This study
XT-D8 UA159 ∆clpP::kan, Kanr This study
XT-Lx1 UA159 carrying pWAR304, Emr , 34
XT-His1 UA159 carrying pSigX-His, Specr 34
XT-His2 XT-D3 carrying pSigX-His, Emr , Specr 34
XT-His3 XT-D7 carrying pSigX-His, Kanr , Specr 34
GF-His1 XT-D8 carrying pSigX-His, Kanr , Specr This study
GF-His2 UA159 carrying pMecA-His, Specr This study
GF-His3 UA159 carrying pClpC-His, Specr This study
GF-His4 UA159 carrying pClpP-His, Specr This study
GF-Ox UA159 pDL277::Pldh-mecA-His, Specr This study
E. coli
DH5α Cloning host Invitrogen
XL1-blue Cloning host Stratagene
BL21(DE3) Recombinant protein expression strain Novagen
BL21(DE3)pLysS Recombinant protein expression strain Novagen
Plasmids
pGEX-6P-1 E. coli expression vector (GST-tag), Ampr GE
pGF-SigX pGEX-6P-1::comX, GST-tag fusion, Ampr This study
pGF-MecA pGEX-6P-1::mecA, GST-tag fusion, Ampr This study
pGF-ClpC pGEX-6P-1::clpC, GST-tag fusion, Ampr This study
pGF-ClpP pGEX-6P-1::clpP, GST-tag fusion, Ampr This study
pET-20b(+) E. coli expression vector (6His-tag), Ampr Novagen
pGF2-SigX pET-20b::comX, His-tag fusion, Ampr This study
pGF2-MecA pET-20b::mecA; His-tag fusion, Ampr; This study
pGF2-ClpC pET-20b::clpC; His-tag fusion, Ampr This study
pGF2-ClpP pET-20b::clpP; His-tag fusion, Ampr This study
pGF2-NTD pET-20b::mecA (aa1–82) , His-tag fusion, Ampr; This study
pGF2-CTD pET-20b::mecA (aa123–240) , His-tag fusion, Ampr; This study
pGF3-P + X pET-20b::Promoter + comX, His-tag fusion, Ampr This study
pDL277 E. coli-Streptococcus shuttle vector, Specr 47
pSigX-His pDL277::Promoter + comX-His, Specr 34
pGF3-PA pET-20b::Promoter + mecA, His-tag fusion, Ampr This study
pMecA-His pDL277::Promoter + mecA-His, Specr This study
pGF3-PC pET-20b::Promoter + clpC, His-tag fusion, Ampr This study
pClpC-His pDL277::Promoter + clpC-His, Specr This study
pGF3-PP pET-20b::Promoter + clpP, His-tag fusion, Ampr This study
pClpP-His pDL277::Promoter + clpP-His, Specr This study
pXT-Pldh pDL277::Promoter of ldh (Pldh), Specr This study
pMecA-Ox pXT-Pldh::MecA-His, Specr This study
pWAR303 A shuttle vector containing promoterless luxAB, Emr 18
pWAR304 pWAR303 having a fusion of PcomX::luxAB, Emr 18