Figure 3
Amphotericin B sensitivity is significantly impacted by biofilm formation. Ten isolates with LBF and HBF were standardised to 1 × 106 cells/mL in RPMI-1640 and grown as biofilms in flat-bottomed 96 well microtitre plates for 24 h. Biofilms were washed with PBS before treated with 2 fold serial dilutions of amphotericin B for 24 h. Biofilms were washed and metabolic activity measured using the XTT metabolic assay with absorbance read at 492 nm. Each isolate was tested in duplicate, on three separate occasions with data represented by mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.