Table 1 Oligonucleotide primers used in this study
Primer ID | Primer Sequence (5’-3’) | Target gene in D39 |
|---|---|---|
SPD0014RTF | GAAGGCATGCTCTGCTTACA | comX |
SPD0014RTR | CGCTTCTGACTTTCCTGCTT | |
SPD0063RTF | ATCCCAATCATCGGTGGTA | strH |
SPD0063RTR | CGGTTCAGGTCTTTTGGTA | |
SPD0065RTF | GGACCTCTTTGTAACAGGAA | bga3 |
SPD0065RTR | CATCTGCCAATTCCTTAGGA | |
SPD0126F | GGAATGAAGGAAGATGATGC | pspA |
SPD0126R | GCCATCTACAGTTGTGTTG | |
SPDRT0144F | GGCGAGAAAGCTTAAGCAGA | rgg |
SPDRT0144R | TTGTGCCCAAACTCATCAAA | |
SPD0344RTF | GCAGAAAAATTGAGCCGAAC | ritR |
SPD0344RTR | CGAAATACGCGCTACCAGAT | |
SPD0420RTF | TGGTGTTTACGCACGTCTTG | pflB |
SPD0420RTR | CATCAACCCCGTAAAGGTCAC | |
SPD0709RTF | TCGTGTGGCTGCCAAGCGTG | gyrB |
SPD0709RTR | GGCTGATCCACCAGCTGAGTC | |
SPD0722RTF | CGTCACCTTCACATGACACC | spxB |
SPD0722RTR | CATGTTGAATGCTCCGTCAC | |
SPD0939RTF | CAAAATTGAAAAATGGGGCTA | rgg/mutR |
SPD0939RTR | GCAAGCTGAGAGACAATCTGC | |
SPD1463RTF | ACTCATTGTAACCAGCGAAGGAGCA | psaA |
SPD1463RTR | CCCAGATGTAGGCACTTGGAACACC | |
SPD1464RTF | AGAATTGGCTGGACTGGACAA | tpxD |
SPD1464RTR | CACCGCACCAACGTTTTTG | |
SPD1499RTF | GGAGTGAGCCAATTTTTGC | nanB |
SPD1499RTR | GCAGGCATAACATCAGCT | |
SPD1504RTF | AGCAACCTCTGGCAAATGAA | nanA |
SPD1504RTR | ATAGTAATCTCTTGGAATT | |
SPD1634F | TCTCGGTGCTCGTATGACAG | galK |
SPD1634R | CACCTGCAACTTCAGCGATA | |
SPD1652RTF | CTTTGGTGCCAAATCTCGTT | piuA |
SPD1652RTR | GCAAGGGTACGGTTGATGAC | |
MP 127 | CCGGGGACTTATCAGCCAACC | pR412 specific |
MP 128 | TACTAGCGACGCCATCTATGTG |