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Table 4 Comparison of raw fold changes in gene expression between S 0 and Na 2 S 2 O 3 -grown cells (mid-log phase) obtained by qRT-PCR in this study

From: Whole-genome sequencing reveals novel insights into sulfur oxidation in the extremophile Acidithiobacillus thiooxidans

Gene

Function

2-ΔΔCT(S0/Na2S2O3)

Sulfide-quinone reductase

sqr

Sulfide quinone reductase

4.14 ± 0.09

sulfur oxygenase reductase

sor

Sulfur oxygenase reductase

4.06 ± 0.01

tetrathionate hydrolase operon

doxD

Thiosulfate:quinone oxidoreductase subunit

3.19 ± 0.01

tetH

Tetrathionate hydrolase

0.96 ± 0.06

Heterodisulfide reductase complex operon

hdrA

Pyridine nucleotide-disulfide oxidoreductase

1.35 ± 0.02

hdrB

Heterodisulfide reductase subunit B

171.37 ± 0.09

hdrC

Iron-sulfur cluster-binding protein

49.69 ± 0.02

Sox operon I

soxA-I

Diheme cytochrome c

48.17 ± 0.01

soxB-I

Sulfate thiol esterase

4.98 ± 0.11

soxX-I

Cytochrome c, class I

13.80 ± 0.08

soxY-I

Covalently sulfur-binding protein

3.89 ± 0.01

soxZ-I

Sulfur compound-chelating protein

150.12 ± 0.06

Sox operon II

soxA-II

Diheme cytochrome c

1.23 ± 0.04

soxB-II

Sulfate thiol esterase

1.42 ± 0.08

soxX-II

Cytochrome c, class I

1.57 ± 0.07

soxY-II

Covalently sulfur-binding protein

0.83 ± 0.04

soxZ-II

Sulfur compound-chelating protein

1.87 ± 0.04

Rhodanese (sulfur transferase)

rhd

Rhodanese

13.04 ± 0.08

  1. Shown are the means of fold change from qRT-PCR of three to four replicates with standard deviations. Genes whose fold change ≥ 1.50 with p-value ≤ 0.05 and fold change ≤ 0.67 with p-value ≤ 0.05 are considered up-regulation and down-regulation, respectively.