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Table 4 Comparison of raw fold changes in gene expression between S 0 and Na 2 S 2 O 3 -grown cells (mid-log phase) obtained by qRT-PCR in this study

From: Whole-genome sequencing reveals novel insights into sulfur oxidation in the extremophile Acidithiobacillus thiooxidans

Gene Function 2-ΔΔCT(S0/Na2S2O3)
Sulfide-quinone reductase
sqr Sulfide quinone reductase 4.14 ± 0.09
sulfur oxygenase reductase
sor Sulfur oxygenase reductase 4.06 ± 0.01
tetrathionate hydrolase operon
doxD Thiosulfate:quinone oxidoreductase subunit 3.19 ± 0.01
tetH Tetrathionate hydrolase 0.96 ± 0.06
Heterodisulfide reductase complex operon
hdrA Pyridine nucleotide-disulfide oxidoreductase 1.35 ± 0.02
hdrB Heterodisulfide reductase subunit B 171.37 ± 0.09
hdrC Iron-sulfur cluster-binding protein 49.69 ± 0.02
Sox operon I
soxA-I Diheme cytochrome c 48.17 ± 0.01
soxB-I Sulfate thiol esterase 4.98 ± 0.11
soxX-I Cytochrome c, class I 13.80 ± 0.08
soxY-I Covalently sulfur-binding protein 3.89 ± 0.01
soxZ-I Sulfur compound-chelating protein 150.12 ± 0.06
Sox operon II
soxA-II Diheme cytochrome c 1.23 ± 0.04
soxB-II Sulfate thiol esterase 1.42 ± 0.08
soxX-II Cytochrome c, class I 1.57 ± 0.07
soxY-II Covalently sulfur-binding protein 0.83 ± 0.04
soxZ-II Sulfur compound-chelating protein 1.87 ± 0.04
Rhodanese (sulfur transferase)
rhd Rhodanese 13.04 ± 0.08
  1. Shown are the means of fold change from qRT-PCR of three to four replicates with standard deviations. Genes whose fold change ≥ 1.50 with p-value ≤ 0.05 and fold change ≤ 0.67 with p-value ≤ 0.05 are considered up-regulation and down-regulation, respectively.