Circular representation of GI-L, PAI-L and potential virulence factors in E. rhusiopathiae strain SY1027 genome. From the outer to inner layers, the circle shows (i) nucleotide positions in kilobases (kb) (black); (ii)Island Viewer-annotated potential genomic islands (GI) are labeled accordingly (red); (iii) tRNAs flanking the potential GI or potential PAI-like regions are labeled accordingly, tRNAs found within 5000-bp flanking regions are marked in dark yellow while that found between5000 to 7000-bp flanking region is marked in grey; (iv) PAIDB-annotated PAI-like regions are labeled accordingly (dark red); (v) VFDB-annotated potential virulence factors (blue); (vi) VFDB-annotated potential virulence factors that might have originated from horizontal gene transfer are classified by their putative origins–plasmid (green) and prophage (orange);(vii) VFDB-annotated potential virulence factors that are previously mentioned in the literature are marked in dark green and their positions boxed in yellow and their gene names labeled accordingly. In cases they are predicted to be horizontally transferred from plasmids, they are boxed in purple. (viii) Other potential virulence factors suggested by previous studies that may be horizontally transferred from plasmids (green); (ix) Other potential virulence factors found in the literature suggested by previous studies, divided into 8 categories – two component system with orientation of 5’histidine kinase-3’ response regulator (cyan), two component system with orientation of 3’histidine kinase-5’ response regulator (orange), surface proteins (brown), antioxidant proteins (vivid green), phospholipases (avocado green), hemolysins (lime), proteins involved in capsular polysaccharide synthesis (pink) and others (pistachio green); (x) percent G + C content (gray). Horizontal transferring genes were annotated by ACLAME database. groEL, VFDB-annotated potential virulence factor that is found within putative PAI-L02, is boxed in red.