Mycobacterium tuberculosis Rv1096 protein: gene cloning, protein expression, and peptidoglycan deacetylase activity

Table 1 Bacteria and plasmids

Bacteria and plasmids	Relevant characteristic(s)	Resource
Strains
E. coli NovaBlue	Used for cloning and propagation of plasmids	Novagen
E. coli ER2566	Used for expression of Rv1096 protein	Novagen
M. smegmatis	mc²155 strain, used for expression of Rv1096 protein and preparation of peptidoglycan	ATCC
E. coli ER2566/Rv1096	E. coli ER2566 carrying pColdII-Rv1096 plasmid	This work
M. smegmatis/Rv1096	M. smegmatis mc²155 carrying pVV2-Rv1096 plasmid	This work
Plasmids
pJET1.2/blunt vector	Carries amp ^R gene; used for cloning PCR product	Fermentas
pColdII-Rv1096	Carries amp ^R gene; used for expression Rv1096 protein in E. coli ER2566	This work
pVV2-Rv1096	Carries kan ^R gene; used for expression of Rv1096 protein in M. smegmatis mc²155	This work

ISSN: 1471-2180