Table 1 Bacteria and plasmids
Bacteria and plasmids | Relevant characteristic(s) | Resource |
|---|---|---|
Strains | ||
E. coli NovaBlue | Used for cloning and propagation of plasmids | Novagen |
E. coli ER2566 | Used for expression of Rv1096 protein | Novagen |
M. smegmatis | mc2155 strain, used for expression of Rv1096 protein and preparation of peptidoglycan | ATCC |
E. coli ER2566/Rv1096 | E. coli ER2566 carrying pColdII-Rv1096 plasmid | This work |
M. smegmatis/Rv1096 | M. smegmatis mc2155 carrying pVV2-Rv1096 plasmid | This work |
Plasmids | ||
pJET1.2/blunt vector | Carries amp R gene; used for cloning PCR product | Fermentas |
pColdII-Rv1096 | Carries amp R gene; used for expression Rv1096 protein in E. coli ER2566 | This work |
pVV2-Rv1096 | Carries kan R gene; used for expression of Rv1096 protein in M. smegmatis mc2155 | This work |