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Table 1 Bacteria and plasmids

From: Mycobacterium tuberculosis Rv1096 protein: gene cloning, protein expression, and peptidoglycan deacetylase activity

Bacteria and plasmids Relevant characteristic(s) Resource
Strains   
E. coli NovaBlue Used for cloning and propagation of plasmids Novagen
E. coli ER2566 Used for expression of Rv1096 protein Novagen
M. smegmatis mc2155 strain, used for expression of Rv1096 protein and preparation of peptidoglycan ATCC
E. coli ER2566/Rv1096 E. coli ER2566 carrying pColdII-Rv1096 plasmid This work
M. smegmatis/Rv1096 M. smegmatis mc2155 carrying pVV2-Rv1096 plasmid This work
Plasmids   
pJET1.2/blunt vector Carries amp R gene; used for cloning PCR product Fermentas
pColdII-Rv1096 Carries amp R gene; used for expression Rv1096 protein in E. coli ER2566 This work
pVV2-Rv1096 Carries kan R gene; used for expression of Rv1096 protein in M. smegmatis mc2155 This work