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Table 5 Genes of the putative T6SSs I and II of Francisella isolate W12-1067

From: Genome sequence and phenotypic analysis of a first German Francisella sp. isolate (W12-1067) not belonging to the species Francisella tularensis

Name (T6SS I)

Peg Nr. (aa)

kDa

Feature

Closest homolog (% aa identity)

OrfD1

950 (92)

9.9

DUF2345, VgrG-like, Rhs family

FTN_0038 (57%)

OrfC1

951 (138)

15.7

SP, lipid attachment site (IglE-like)

FTN_0039 (66%)

OrfB1

952 (1097)

127.1

SP, TM domain (PdpB-like/TssM)

FTN_0040 (58%)

OrfA1

953 (735)

84.6

Cl*

FTN_0041 (63%)

Orf48

954 (48)

5.8

SP

---

ImpB1

955 (179)

20.8

homologous to IglA/TssB

FTN_0042 (96%)

ImpC1

956 (508)

57.7

homologous to IglB/TssC

FTN_0043 (91%)

OrfE1

957 (214)

23.1

helical bundle domain (putative TssD)

FTN_0044 (77%)

OrfF1

958 (356)

41.3

Cl

FTN_0045 (51%)

OrfG1

059 (406)

47.3

Cl, T6SS-associated like protein

FTN_0048 (72%)

OrfH1

960 (274)

31.9

C-terminal TM domain

FTN_0049 (63%)

OrfI1

961 (501)

58.6

Cl

FTN_0050 (72%)

OrfJ1

962 (203)

23.8

DUF2077 (putative DotU/TssL)

FTN_0051 (63%)

OrfK1

963 (126)

13.1

DUF4280

FTN_0054 (82%

Orf374

964 (374)

43.3

Cl

Hyp. protein (33%) Flavobacterium

IglD3

1538 (398)

46.1

DUF876 (TssK)

IglD (49%) F-TX077308

Name (T6SS II)

Peg Nr.

kDa

Feature

Closest homolog (% aa identity)

ImpB2

1376 (177)

19.9

Cl, homologous to IglA/TssB, DUF770

FTN_0042 (42%)

ImpC2

1375 (493)

55.5

Cl, homologous to IglB/TssC, COG3517

FTN_0043 (47%)

Orf204

1374 (204)

22.4

Cl,

---

OrfG2

1373 (405)

47.1

Cl, IglD-like

F7308_1914 (28%)

Orf368

1372 (368)

43.4

Cl

---

OrfL

1371 (629)

74.6

Hypothetical protein

F7308_1916 (33%)

Orf96

1370 (96)

11.1

2 TM domains

Hyp. protein (49%) Prevotella histicola

OrfB2

1369 (928) [1020]

106.3

Cytoplasmic membrane, SP

FTN_0040 (20%)

Orf457

1368 (457)

53.4

Hypothetical protein

---

Orf254

1367 (254)

30.3

Cl

---

OrfC2

1366 (146)

17

SP

FTN_0039 (24%)

OrfJ2

1365 (195)

23.1

DUF2077 (putative DotU/TssL)

DotU, (29%) Desulfonatronospira

OrfD2

1364 (111)

12.4

Cl (DUF2345, vgrG)

---

ORF705

1363 (705)

77.7

Cl

---

  1. *determined by using “psortb” (http://www.psort.org/psortb); Cl, cytoplasmic localization; TM, transmembrane domain; SP, signal peptide; (aa), number of amino acids.