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Table 2 Primers used in the construction and confirmation of recombinant pCT plasmids

From: Functional genomics to identify the factors contributing to successful persistence and global spread of an antibiotic resistance plasmid

pCT gene

Description

Primer sequence

Amplimer size (bp) (WT/inactivation)

Sigma factor pCT_066

Confirmation forward

ACAGCGTCTTCTCGTATCCA

1289/1675

Confirmation reverse

GTTCTTCCAGCTGACGTAAC

1289/1675

Recombination 1

GGAGGGCGTCTCGCTAAAAAAACTTACTCAAACACATCAAGTGTAGGCTGGAGCTGCTTC

1574

Recombination 2

GCATTACTTTTTATTCTCGTGAGACTCAAGGTCATTCGGTGGGAATTAGCCATGGTCCAT

1574

rci

Confirmation forward

AAGGTCATCTGCAGGAGT

945/1867

Confirmation reverse

GTGTCGCAGCAACAATA

945/1867

Recombination 1

GGGGGACATGCCGTATGAATCCTGTTGAACTGGTCCGAAAGTGTAGGCTGGAGCTGCTTC

1574

Recombination 2

GCAGTGTCACGACAAACAGCCCGTTTCTGCACCCGACAGTGGGAATTAGCCATGGTCCAT

1574

pilS

Confirmation forward

GCGGAAGGAAGTGAGCATAA

722/2053

Confirmation reverse

CAGTGACATGCTGAAGCAGT

722/2053

Recombination 1

TGGTGACCAGATCAATACAGTTTTTCTTCGGCACATTGCTGTGTAGGCTGGAGCTGCTTC

1574

Recombination 2

AACCTGCAGACAATCGCCACCAAAATGAAAGCCCAGAAAGGGAATTAGCCATGGTCCAT

1574

traY

Confirmation forward

GGAGAGTCCGGTCTGTATGA

2423/2138

Confirmation reverse

TGCAACCAGTGTGGTACAG

2423/2138

Recombination 1

GTATCCTGGTCTGCCTGTTACTGATGAGTACCATTGCAGCGTGTAGGCTGGAGCTGCTTC

1574

Recombination 2

CGGCACAAAACAGCAAAAACAGCAGGAAGTAGAGTGGTGGGGGAATTAGCCATGGTCCAT

1574

pndACB

Confirmation forward

AAGGATTGTGGCGGACAGGA

486/1288

Confirmation reverse

TGATGACGCACAGGACGGAA

486/1288

Recombination 1

CCCAGGCGATTTTTTTATCAATCAACCCAGGGCCCACTGTGTGTAGGCTGGAGCTGCTTC

1574

Recombination 2

ATTGAGGTCAGCCTTCGCAACAATCCGGCGGCAGATGTCCGGGAATTAGCCATGGTCCAT

1574

parB

Confirmation forward

TATTAAAAATAACGCGGCGG

663/1872

Confirmation reverse

GCAAAGTATCACACTGCCAAAA

663/1872

Recombination 1

GGAGCGGCGGGAGAGTATAGTCATTATTGTAGTCCGGGTAGTGTAGGCTGGAGCTGCTTC

1574

Recombination 2

CTTTTCACTCACCATTATTTTTTCCGCTTCTCTCTGTGCCGGGAATTAGCCATGGTCCAT

1574