Table 2 Primers used for amplification and sequencing of M.tuberculosis clinical strains
Gene | Primer name (position*) | Primer sequence (5′→3′) | Annealing temp (°C) | PCR product size (bp) | Purpose | Reference |
|---|---|---|---|---|---|---|
rrs | F-rrs (-44) | 5′-TTCTAAATACCTTTGGCTCCCT-3′ | 51 | 1,680 | PCR/Seq | [42] |
R-rrs (1,636) | 5′-TGGCCAACTTTGTTGTCATGCA-3′ | 53 |  | PCR/Seq | [42] | |
F-rrs1 (554) | 5′-CTGGGCGTAAAGAGCTCGTA-3′ | 54 |  | Seq | This study | |
F-rrs2 (1,114) | 5′-GTTGCCAGCACGTAATGGTG-3′ | 54 |  | Seq | This study | |
R-rrs1 (483) | 5′-TCCACCTACCGTCAATCCGA-3′ | 54 |  | Seq | This study | |
R-rrs2 (1,073) | 5′-ATCTCACGACACGAGCTGAC-3′ | 54 |  | Seq | This study | |
eis (Rv2416c) | F-Rv2417c (-316) | 5′-GCGGTGCATCACGTCGCCGA-3′ | 60 | 1,661 | PCR/Seq | This study |
R-eis-Rv2415c (1,345) | 5′-GCAACGCGATCCGCGAGTGC-3′ | 60 |  | PCR/Seq | This study | |
F-eis1 (247) | 5′-AGTTTCGTCGCGGTGGCGCC-3′ | 60 |  | Seq | This study | |
F-eis2 (816) | 5′-GGACCCGTTACCCCACCTGC-3′ | 60 |  | Seq | This study | |
R-eis1 (240) | 5′-GGCGGTCGGGAGCACCACTT-3′ | 60 |  | Seq | This study | |
R-eis2 (769) | 5′-TCAGGGCCCGCCACAACGCA-3′ | 60 |  | Seq | This study | |
tap (Rv1258c) | F-Rv1259 (-496) | 5′-CAGGCCGGCCCTATGCAGTG-3′ | 60 | 1,847 | PCR/Seq | This study |
R-Rv1257c (1,351) | 5′-CGGTCTTGCCGGTAGCCGTC-3′ | 60 |  | PCR/Seq | This study | |
F-tap1 (41) | 5′-TCGCAACGCTGATGGCGGCC-3′ | 60 |  | Seq | This study | |
F-tap2 (641) | 5′-AGGGGCTGCGCTTCGTCTGG-3′ | 60 |  | Seq | This study | |
R-tap1 (210) | 5′-CCCGAAGTAGTCGACCGCGG-3′ | 60 |  | Seq | This study | |
R-tap2 (862) | 5′-GACGGGGAACGCGGATAGCC-3′ | 60 |  | Seq | This study | |
whiB7 (Rv3197A) | F URT-whiB7 (-451) | 5′-GCTGGTTCGCGGTCGGACCT-3′ | 60 | 550 | PCR/Seq | This study |
R whiB7 (99) | 5′-CGGGGTATCGGCGAACCACA-3′ | 58 |  | PCR/Seq | This study | |
tlyA (Rv1694) | F-tlyA (1) | 5′-GTGGCACGACGTGCCCGCGT-3′ | 62 | 807 | PCR/Seq | This study |
R-tlyA (807) | 5′-CTACGGGCCCTCGCTAATCG-3′ | 58 |  | PCR/Seq | This study |