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Table 2 Growth of Castellaniella defragrans 65Phen transposon insertion mutants in liquid medium

From: The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen

Inactivated gene Annotation Length [b] Insertion positions [b] Growth substrate*
Acetate Limonene β-Myrcene Further substrates
Initial oxidation
CDM25290 CtmA Limonene dehydrogenase, alpha subunit 1698 133 + - - Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)
CDM25290 CtmA Limonene dehydrogenase, alpha subunit 1698 1188 + - - Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)
CDM25289 CtmB Limonene dehydrogenase, beta subunit 1650 38 + - - Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)
CDM25289 CtmB Limonene dehydrogenase, beta subunit 1650 559 + - - Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)
CDM25286 CtmE Ferredoxin, 2Fe-2S 324 111 + - - Perillyl alcohol (+), α-phellandrene (-), α-pinene (-), β-pinene (-)
Ring cleavage and β-oxidation
CDM23589 Electron transfer flavoprotein:ubiquinone oxidoreductase 1647 138 + (+) (+) Perillic acid ((+))
CDM25250 MrcF Perillyl-CoA hydratase 1239 18 + - - α-phellandrene (-)
CDM25253 MrcC 2,4-dienoyl-CoA reductase 888 841 + - - α-phellandrene (-)
CDM25297 Hypothetical protein 1023 34 + - -  
CDM258488 Electron transfer protein 888 385 + - - Perillic acid (-)
CDM25923 Enoyl-CoA hydratase 777 461 + - -  
Methylcitrate cycle
CDM25082 2-methylcitrate dehydratase 1452 262 + - -  
CDM25081 2-methylcitrate synthase 1239 -18 + (+) +  
Other functions
CDM23676 Molybdenum transport system protein 865 47 + - - Perillyl aldehyde (-)
CDM25260 RND efflux transporter, periplasmic component 1356 24 + (+) (+) Perillic acid ((+))
  1. * + growth like wild type; - no growth; (+) decreased growth rate and maximum density compared to wild type.