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Figure 3 | BMC Microbiology

Figure 3

From: The ColRS signal transduction system responds to the excess of external zinc, iron, manganese, and cadmium

Figure 3

ColR-regulated genes respond to excess of zinc, iron, manganese and cadmium. β-galactosidase activities measured in P. putida wild-type (wt) and colS-deficient strain (colS) carrying the transcriptional fusions of PP0268 or PP0903 promoters with lacZ in the plasmid p9TTBlacZ. Bacteria were grown in LB medium and in LB containing either 0.6 mM ZnSO4, 0.15 mM FeSO4, 0.5 mM MnCl2, 0.1 mM CoCl2, 2 mM CuSO4, 0.5 mM NiSO4 or 0.2 mM CdSO4. Data (means with 95% confidence intervals) of at least three independent experiments are presented. Asterisks indicate statistically significant differences (p < 0.05, two-way ANOVA with post-hoc Tukey’s Unequal N HSD test) between values obtained in LB and in LB supplemented with metal salt.

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