Table 2 Oligonucleotides used in this study
Name | 5′-3′ sequencea | Used for | References |
|---|---|---|---|
prRhlG1 | attatgagctc CATCCTGTTCGTCCTGTTC (Sac I) | cloning of rhlG promoter | This study |
prRhlG2 | atattactagt GGGAGACCAGCCTACGAT (Spe I) | cloning of rhlG promoter | This study |
rhlG33 | GGATGCTGGCGAAGGAACT | qRT-PCR | This study |
rhlG34 | GTCATGCGGCTCGGAAAG | qRT-PCR | This study |
16sFad1 | CAGGATTAGATACCCTGGTAGTCCAC | qRT-PCR | [35] |
16sRad2 | GACTTAACCCAACATCTCACGACAC | qRT-PCR | [35] |
rhlGko1 | tatagaaTTC GTCGAGCACTACCTGTTG (Eco RI) | Knock out | This study |
rhlGko2 | tatactGCAG TTGCTGGATGCAGGA (Pst I) | Knock out | This study |
rhlGko3 | tatactgcaG CCTACATGACCGGCAAC (Pst I) | Knock out | This study |
rhlGko4 | atataagcTT GGTCGAGCCGCTGAT (Hin dIII) | Knock out | This study |
PA3388ko1 | tatagaaTTC ATCTGCGCACGTGAC (Eco RI) | Knock out | This study |
PA3388ko2 | tatatctAGA AACGCTGTGGGTCATG (Xba I) | Knock out | This study |
PA3388ko3 | ttattctaGA TATCAAGCCCTACGTACCCTAC (Xba I) | Knock out | This study |
PA3388ko4 | atttaagcTT CCGTGTACTGCATCTTTATCA (Hin dIII) | Knock out | This study |
PA3388ko5 | ttattctgcaG ATATCAAGCCCTACGTACCCTAC (PstI) | Knock out | This study |
Gsp1G | TGCGTCTTGAGTATTCTTCA | 5′-RACE PCR | This study |
Gsp2G | GCCCTACCGTATAGAGAAAA | 5′-RACE PCR | This study |
NesG | CCGTAATTCGTTATTTCCAT | 5′-RACE PCR | This study |