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Table 1 Bacterial strains and plasmids used in this study

From: The Pseudomonas aeruginosa rhlG and rhlAB genes are inversely regulated and RhlG is not required for rhamnolipid synthesis

Strain or plasmid Description Reference(s) or source
P. aeruginosa   
PAO1 Plasmid-free strain [31]
PAO6358 rpoN mutant [24]
PDO100 rhlI mutant [25]
PAOGAB rhlG mutant This study
PAOFDO PA3388 mutant This study
PAOJBB rhlG/PA3388 mutant This study
PAOU algU mutant [21]
Escherichia coli   
Top10 Electrocompetent cells Invitrogen
S17.1 RecA pro (RP4-2Tet::Mu Kan::Tn7) [26]
Plasmids   
pBBR1MCS-5 Cloning vector, GmR [32]
pAB133 Promoter-less luxCDABE operon cloned in pBBR1MCS-5, GmR [17]
pAB134 rhlG promoter cloned in pAB133, GmR This study
pEX100Tlink P. aeruginosa suicide vector, AmpR [26]
pUCGmlox AmpR, GmR, pUC18-based vector containing the lox flanked aacC1 [26]
pCM157 cre expression vector, TcR [33]
pGAB10 Deleted rhlG cloned in pEX100Tlink, AmpR This study
pFAB1 Deleted PA3388 cloned in pEX100Tlink, AmpR This study
pJBB1 Deleted rhlG-PA3388 operon cloned in pEX100Tlink, AmpR This study
pGAB10.14 lox flanked aacC1 from pUCGmlox cloned in pGAB10, AmpR GmR This study
PFAB1.13 lox flanked aacC1 from pUCGmlox cloned in pFAB1, AmpR GmR This study
pJBB11 lox flanked aacC1 from pUCGmlox cloned in pJBB, AmpR GmR This study
pGAB Complementation, rhlG cloned in pBBR1MCS-5, GmR This study