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Figure 6 | BMC Microbiology

Figure 6

From: Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide

Figure 6

Experimental EPR spectra (black line) and theoretical fits (red line) of spin-label 5-DSA in Leishmania membrane. The experiment was conducted at 26°C for samples untreated and treated with parthenolide at the indicated concentrations. EPR spectra were simulated with the NLLS fitting program, and the values of the parameter rotational correlation time, τ C , obtained from the fit for each spectrum are indicated on a nanosecond scale. The EPR parameter 2A//is the separation in magnetic-field units between the first and last resonance lines of the spectrum. The vertical lines indicate the 2A//for the control samples, and the smaller vertical lines illustrate the increase in 2A//for the sample treated with 9 × 109 molecules/cell. The measured 2A//values and τC values indicate that the presence of parthenolide significantly reduced lipid fluidity. The estimated experimental errors for the 2A//and τC parameters are 0.5 G and 1.0 ns, respectively.

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