Figure 3
From: Cell death in amastigote forms of Leishmania amazonensis induced by parthenolide
Scanning (A-C) and transmission (D-I) electron microscopy of axenic amastigotes of L. amazonensis treated with parthenolide. Amastigotes were incubated for 72 h in the absence (A and D) or presence (B, C, F-I) of the IC50 (1.3 μM) of parthenolide. For transmission electron microscopy, the treatment of amastigotes was also accomplished using the IC50 of amphotericin B as a reference drug that acts on the cytoplasmic membrane (E). The arrows indicate plasma membrane blebs or loss of membrane integrity, and the asterisks indicate vesicles located in the cytoplasm or flagellar pocket. n, nucleus; f, flagellum; fp, flagellar pocket; m, mitochondrion; k, kinetoplast. Scale bars = 1 μm.