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Table 2 Genes and sequencing primers used

From: A novel multi-locus sequence typing (MLST) protocol for Leuconostoc lactis isolates from traditional dairy products in China and Mongolia

Gene

Protein

PCR primers

Amplicon size (bp)

Location*

pyrG

CTP synthase

5′-AGCAAACACCCAAGAACG-3′

598

481322 to 482935

  

5′-TGGTGAAGCGAAGACAAA-3′

  

rpoB

DNA-directed RNA polymerase subunit beta

5′-CACTGTGCGGTCGTCTTCC-3′

608

1798123 to 1801731

  

5′-GCGTTCTCCTGGTATCTATT-3′

  

groEL

Chaperonin GroEL

5′-CGGTGATAAGGCTGCTGT-3′

892

1734716 to 1736335

  

5′-TTTGTTGGGTCCACGATA-3′

  

recA

Recombinase A

5′-GGAGTCGTTTCTGGGTTAC-3′

550

555064 to 556221

  

5′-GTTGCTTTAGGCGTTGGTG-3′

  

uvrC

Excinuclease ABC subunit C

5′-AGAAATACAAGCCGTACTACAA-3′

560

483053 to 484852

  

5′-TCTTCATCAGCGGAACCAA-3′

  

carB

Carbamoyl phosphate synthase large subunit

5′-ATGGGTTGTGGGAGTTGTA-3′

833

1202174 to 1205353

  

5′-ACTTGTTGCGTCGTGGTGT-3′

  

murC

UDP-N-acetylmuramate-L-alanine ligase

5′-TTTCATAGGCGAACTCAT-3′

619

679802 to 681136

  

5′-GTGCCATTGTTTGGTCAG-3′

  

pheS

Phenylalanyl-tRNA synthetase subunit alpha

5′-TTTCTTAGGTTTAGGCTTTG-3′

665

406737 to 407813

  

5′-CCTTTCGGTTAAATTGTGA-3′

  
  1. *Positions correspond to the complete genome sequence of Leu. mesenteroides subsp. mesenteroides ATCC 8293.