Figure 3

Phosphorylation of c-Fos and c-Jun in EV71-infected iDCs. (A and D) The western blot results of cell lysates collected at indicated times of iDCs infected with EV71 (MOI = 5) for 24 h using antibodies against total and phosphorylated c-Fos and c-Jun. (B and E) The western blot results of cell lysates collected at indicated times of iDCs pretreated with SP600125 (20 μM) for 1 h and infected with EV71 (MOI = 5) for 24 h using antibodies against total and phosphorylated c-Fos and c-Jun. (C and F) The western blot results of cell lysates collected at indicated times of iDCs pretreated with SB203580 (20 μM) for 1 h and infected with EV71 (MOI = 5) for 24 h using antibodies against total and phosphorylated c-Fos and c-Jun. The intensities of phosphorylated c-Fos and c-Jun were quantitated and normalized as described. The data were expressed as mean ± SE from three independent experiments and analyzed by one-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001).