Table 4 Comparison of the results obtained from blood of patients with clinical symptoms of sepsis by the method of blood culture, the nested multiplex qPCR and the multiplex qPCR methods in the parentheses and the internal inhibition control for the β-actin gene
From: A novel, nested, multiplex, real-time PCR for detection of bacteria and fungi in blood
Blood culture | Nested-multiplex-qPCR (n = 102) | Total positive samples | β-actin gene | ||||
|---|---|---|---|---|---|---|---|
(Multiplex-qPCR) | |||||||
(n = 102) | Gram positive | Gram negative | Yeast | Filamentous fungi | (n = 102) | ||
43 | 51 | 1 | 0 | ||||
(16) | (18) | (0) | (0) | 71 | |||
Positive | 19 | (18) | 102 | ||||
42.1 | 50.0 | 1 | 0 | ||||
(15.7) | (17.6) | (0) | (0) | 69.6 | |||
% | 18.6 | (17.6) | 100.0 | ||||