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Table 1 Sequences of primers and probes utilized in the study

From: A novel, nested, multiplex, real-time PCR for detection of bacteria and fungi in blood

Amplification

Oligonucleotide

Sequence 5′ - 3′

Origin

Target sequences

I

*EXT_BAC_F

kGCGrACGGGTGAGTAA

in this study

16S rRNA

*EXT_BAC_R

CGCATTTCACCGCTA

in this study

*EXT_FUN_F

AATTGACGGAAGGGCACC

in this study

18S rRNA

*EXT_FUN_R

TTCCTCGTTGAAGAGCAA

in this study

II

**FUN_F

TTGGTGGAGTGATTTGTCTGCT

[11]

18S rRNA

**FUN_R

TCTAAGGGCATCACAGACCTG

Candid_probe

FAM-TTAACCTACTAAATAGTGCTGCTAGC-BHQ1

Asperg_probe

TexasRed-TCGGCCCTTAAATAGCCCGGTCCGC-Eclipse

**GN/GP_F

GACTCCTACGGGAGGC

[10]

16S rRNA

**GN/GP_R

GCGGCTGCTGGCAC

GP_Probe

Hex- CTGAyssAGCAACGCCGCG –TAMRA (Q)

GN_Probe

Cy5 –CCTGAysCAGCmATGCCGCG- BHQ-2

β-actin gene

F

GCCAGTGCCAGAAGAGCCAA

[16]

β-actin gene

 

R

TTAGGGTTGCCCATAACAGC

  
  1. *External primers; **internal primers; k = G/T; r = A/G.