From: A novel, nested, multiplex, real-time PCR for detection of bacteria and fungi in blood
Amplification | Oligonucleotide | Sequence 5′ - 3′ | Origin | Target sequences |
---|---|---|---|---|
I | *EXT_BAC_F | kGCGrACGGGTGAGTAA | in this study | 16S rRNA |
*EXT_BAC_R | CGCATTTCACCGCTA | in this study | ||
*EXT_FUN_F | AATTGACGGAAGGGCACC | in this study | 18S rRNA | |
*EXT_FUN_R | TTCCTCGTTGAAGAGCAA | in this study | ||
II | **FUN_F | TTGGTGGAGTGATTTGTCTGCT | [11] | 18S rRNA |
**FUN_R | TCTAAGGGCATCACAGACCTG | |||
Candid_probe | FAM-TTAACCTACTAAATAGTGCTGCTAGC-BHQ1 | |||
Asperg_probe | TexasRed-TCGGCCCTTAAATAGCCCGGTCCGC-Eclipse | |||
**GN/GP_F | GACTCCTACGGGAGGC | [10] | 16S rRNA | |
**GN/GP_R | GCGGCTGCTGGCAC | |||
GP_Probe | Hex- CTGAyssAGCAACGCCGCG –TAMRA (Q) | |||
GN_Probe | Cy5 –CCTGAysCAGCmATGCCGCG- BHQ-2 | |||
β-actin gene | F | GCCAGTGCCAGAAGAGCCAA | [16] | β-actin gene |
R | TTAGGGTTGCCCATAACAGC |