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Table 1 Standard S. pneumoniae amplification and sequencing primers proposed by Enright and Spratt[11]

From: Effectiveness of the standard and an alternative set of Streptococcus pneumoniae multi locus sequence typing primers

Typing gene

Primer sequences

Annealing temperature °C

aroE shikimate dehydrogenase

F: 5′-GCCTTTGAGGCGACAGC

55

R: 5′-TGCAGTTCA(G/A)AAACAT(A/T)TTCTAA

gdh glucose-6-phosphate dehydrogenase

F: 5′-ATGGACAAACCAGC(G/A/T/C)AG(C/T)TT

55

R: 5′-GCTTGAGGTCCCAT(G/A)CT(G/A/T/C)CC

gki glucose kinase

F: 5′-GGCATTGGAATGGGATCACC

60

R: 5′-TCTCCCGCAGCTGACAC

recP transketolase

F: 5′-GCCAACTCAGGTCATCCAGG

65

R: 5′-TGCAACCGTAGCATTGTAAC

spi signal peptidase I

F: 5′-TTATTCCTCCTGATTCTGTC

50

R: 5′-GTGATTGGCCAGAAGCGGAA

xpt xanthine phosphoribosyltransferase

F: 5′-TTATTAGAAGAGCGCATCCT

55

R: 5′-AGATCTGCCTCCTTAAATAC

ddl D-alanine-D-alanine ligase

F: 5′-TGC(C/T)CAAGTTCCTTATGTGG

65

R: 5′-CACTGGGT(G/A)AAACC(A/T)GGCAT