Simulation of human digestion in shake flasks. (a) Survival of L. crispatus L1 to gastric juices (pH 2.0, pepsine 3 g∙l−1). Response of different doses of bacteria, high (1.8 · 109 cells∙ml−1) and low (6.0 · 108 cells∙ml−1), to the treatment. (b) Survival of L. crispatus L1 to pancreatic juices (pH 4.0, pancreatine 2 g∙l−1, Oxgall in different concentrations). Effect of two different concentrations of bile salts on the viability of 1.0 · 109 cells∙ml−1.The asterisks indicate a statistically significant difference between samples with P < 0.01.