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Table 2 Bacterial concentration of different microbial groups quantified by specific qPCR in luminal (L) (n = 3) and mucosal (M) (n = 6) samples of the HMI module during control and treatment at time 0, 24 and 48 h

From: The HMI™ module: a new tool to study the Host-Microbiota Interaction in the human gastrointestinal tract in vitro

  

Control (A)

Treatment (B)

  

0 h

24 h

48 h

0 h

24 h

48 h

  

L

L

M

L

M

L

L

M

L

M

Total Bacteria

Avg.

2.46 × 1010

1.31 × 1010

5.71 × 108

9.08 × 109

6.35 × 108

6.35 × 10 9

6.27 × 10 9

2.43 × 10 8

7.79 × 109

2.31 × 10 7

 

Std. Dev.

1.12 × 109

1.53 × 108

2.83 × 108

4.77 × 108

8.44 × 108

3.14 × 108

7.54 × 107

1.75 × 108

2.29 × 108

2.56 × 106

Bacteroidetes

Avg.

7.60 × 109

6.29 × 109

5.25 × 108

4.58 × 109

2.78 × 108

1.41 × 10 9

4.50 × 109

9.82 × 10 7

1.13 × 10 10

6.59 × 107

 

Std. Dev.

1.23 × 109

2.77 × 109

3.60 × 108

1.20 × 109

3.65 × 108

1.83 × 108

6.96 × 108

6.07 × 107

1.79 × 109

3.44 × 107

Firmicutes

Avg.

1.65 × 109

1.64 × 108

2.08 × 107

2.85 × 108

1.67 × 107

7.88 × 10 8

4.29 × 10 8

3.65 × 106

5.43 × 10 8

9.65 × 10 5

 

Std. Dev.

2.79 × 108

1.02 × 107

3.80 × 106

2.52 × 107

3.20 × 106

7.21 × 107

3.96 × 107

1.60 × 106

4.11 × 107

7.41 × 105

Bifidobacteria

Avg.

9.39 × 108

2.73 × 108

3.35 × 108

3.24 × 108

8.49 × 106

1.26 × 10 8

3.79 × 108

1.25 × 10 6

4.43 × 10 8

3.37 × 10 5

 

Std. Dev.

1.23 × 108

2.65 × 107

5.09 × 107

2.97 × 107

9.80 × 105

2.89 × 107

1.40 × 108

1.38 × 105

2.44 × 107

1.74 × 105

Lactobacilli

Avg.

1.88 × 107

3.86 × 106

1.30 × 105

6.81 × 105

3.45 × 102

8.06 × 10 5

1.77 × 10 5

1.45 × 10 3

1.37 × 106

5.85 × 10 4

 

Std. Dev.

3.47 × 106

3.45 × 105

7.75 × 104

5.40 × 105

3.89 × 102

1.69 × 105

1.54 × 105

1.67 × 103

2.52 × 105

7.86 × 104

  1. Data for L are expressed as 16S rRNA gene copies/mL of SHIME suspension; those for M correspond to 16S rRNA gene copies cm−2 of simulated gut wall. Values in bold indicate samples from the treatment period which are significantly higher than the control at the same sampling time, according to a Student’s two-tailed t test (p < 0.05). Values in italics are significantly lower.