Immunoblotting of infected hamster urine by anti-HADH antisera. (A) Rabbit anti-rHADH serum was able to detect the 52 kDa protein (*) in the urine of infected hamsters (n = 3). Each lane contained 5 μg of protein. (B) It was revealed, by serial dilution of urine, that HADH increased in the said specimen during the seventh day post infection. These experiments were repeated three times, and the representative data are shown in this figure.