SDS-PAGE and immunoblotting of recombinant leptospiral HADH. (A) The rHADH with His-tag was produced by E. coli and purified by cobalt resin. In total, 1 μg of the protein was run by SDS-PAGE and CBB staining. (B) Anti-His-tag antibody and (C) anti-L. interrogans pAb detected the protein. Sharp signs indicate recombinant protein bands of 52 kDa. These experiments were repeated three times, and the representative data are shown in this figure.