SDS-PAGE and immunoblotting of hamster urine protein during Leptospira infection. (A) Compositions of hamster urinary proteins were compared according to infection periods. Hamster urine was collected and prepared for SDS-PAGE. The urine of three hamsters was mixed for each infection period. The protein content of each sample was 5 μg. After separation with SDS-PAGE, the gel was stained by silver staining. (B) The anti-L. interrogans pAb recognized leptospiral proteins in infected-hamster urine by immunoblotting. These experiments were repeated three times, and the representative data are shown in this figure.