Characterization of AcrD, a Resistance-Nodulation-Cell Division-type multidrug efflux pump from the fire blight pathogen Erwinia amylovora

Table 3 Relative fold-changes in mRNA transcripts of acrA and acrD after inoculation of Erwinia amylovora Ea1189 on apple rootstocks MM106 and immature pear fruit slices, respectively ^a

^aTotal RNA was isolated from bacterial cells recovered from infected plant tissues. Transcript abundance of acrA and acrD was determined by quantitative RT-PCR and was compared to RT-PCR signal from cells grown in LB broth to an OD₆₀₀ of 0.5.
^bBacteria were re-isolated from infected shoots of apple rootstock 1, 4 and 7 days post inoculation (dpi).
^cBacteria were re-isolated from infected immature pears 12 hours post inoculation (hpi).

ISSN: 1471-2180