Characterization of AcrD, a Resistance-Nodulation-Cell Division-type multidrug efflux pump from the fire blight pathogen Erwinia amylovora

Table 2 Virulence assay on apple rootstock MM 106

Strain	Re-isolated bacterial cells^a
	1 dpi	5 dpi
Ea1189	2.5 × 10⁶ ± 1.1 × 10⁶	4.7 × 10⁸ ± 1.1 × 10⁸
Ea1189.acrD	6.1 × 10⁶ ± 4.7 × 10⁶	3.5 × 10⁸ ± 1.1 × 10⁸

^aBacteria were inoculated by prick technique in the shoot tips with an inoculum of 5 × 10⁶ CFU/shoot. Establishment of a population of Erwinia amylovora Ea1189 and acrD mutant (CFU/shoot) was determined 1 and 5 days post inoculation (dpi), respectively.