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Table 1 Antimicrobial susceptibility profiles from an E. amylovora wild-type strain, acrD mutant, acrB mutant complemented with AcrD-overexpression plasmids pBlueSK.acrD ( acrD under control of P lac ) and pBlueKS.acrD-ext ( acrD under control of P lac and its native promoter P acrD ) and acrB mutant complemented with control plasmid pBlueSK.acrD ( acrD in opposite orientation to P lac )

From: Characterization of AcrD, a Resistance-Nodulation-Cell Division-type multidrug efflux pump from the fire blight pathogen Erwinia amylovora

Drug

MIC (μg/ml)a

 

Ea1189

Ea1189.acrD

Ea1189-3 (pBlueSK.acrD)

Ea1189-3 (pBlueKS.acrD)

Ea1189-3 (pBlueKS.acrD-ext)

   

Plac < < acrD

Plac> > acrD

Plac, PacrD> > acrD

Antimicrobials

     

Benzalkonium chloride

12.5

12.5

1.2

1.2

ND

Chloramphenicol

3.1

ND

1.2

1.2

1.2

Clotrimazole

> 1000

> 1000

6.2

12.5

25

Fusaric acid

500

500

500

500

500

Fusidic acid

250

250

3.1

6.2

25

Genistein

> 5000

> 5000

62.5

62.5

62.5

Josamycin

125

125

3.1

3.1

3.1

Luteolin

> 5000

> 5000

15.63

15.6

125

Naladixic acid

2.5

2.5

1.2

1.2

1.2

Naringenin

5000

5000

312

312

312

Nitrofurantoin

25

12.5

12.5

12.5

12.5

Norfloxacin

0.63

0.63

0.03

0.03

0.03

Novobiocin

250

250

6.2

25

100

Phloretin

5000

5000

625

625

625

Rifampicin

12.5

12.5

12.5

12.5

12.5

Tetracycline

1.5

1.5

1.2

1.2

1.2

Aminoglycosides

     

Amikacin

2.5

2.5

2.5

2.5

2.5

Gentamicin

2.5

2.5

2.5

2.5

2.5

Hygromycin B

100

100

62.5

125

125

Streptomycin

2.5

2.5

2.5

2.5

2.5

Tobramycin

2.5

2.5

2.5

2.5

2.5

Macrolids

     

Azithromycin

0.31

0.31

0.63

0.63

0.63

Clarithromycin

0.31

0.31

0.31

0.31

0.31

Erythromycin

0.63

0.31

0.16

0.16

0.16

Roxithromycin

1.25

1.25

0.16

0.16

0.16

Heavy metals

     

Cadmium acetate

12.5

12.5

25

50

50

Cobalt (II) chloride

625

625

1250

1250

1250

Copper (II) sulfate

1250

1250

1250

1250

1250

Nickel (II) chloride

1250

1250

2500

2500

2500

Silver nitrate

12.5

6.2

6.2

6.2

6.2

Sodium tungstate

125000

62500

125000

125000

125000

Zinc sulfate

156

156

156

312

312

Dyes

     

Acriflavine

50

50

6.2

6.2

6.2

Crystal violet

3.1

3.1

2.5

2.5

2.5

Ethidium bromide

250

250

3.1

3.1

6.2

Rhodamine 6G

> 100

> 100

3.1

3.1

3.1

Detergents

     

Bile salt

5000

5000

625

1250

5000

Deoxycholate

> 1000

> 1000

312

1250

2500

SDS

> 1000

> 1000

62.5

125

125

  1. aMIC values were determined by the 2-fold dilution assay in three or more independent experiments with similar results. Boldface numbers indicate a higher or lower MIC. ND, not determined.