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Table 1 Antimicrobial susceptibility profiles from an E. amylovora wild-type strain, acrD mutant, acrB mutant complemented with AcrD-overexpression plasmids pBlueSK.acrD ( acrD under control of P lac ) and pBlueKS.acrD-ext ( acrD under control of P lac and its native promoter P acrD ) and acrB mutant complemented with control plasmid pBlueSK.acrD ( acrD in opposite orientation to P lac )

From: Characterization of AcrD, a Resistance-Nodulation-Cell Division-type multidrug efflux pump from the fire blight pathogen Erwinia amylovora

Drug MIC (μg/ml)a
  Ea1189 Ea1189.acrD Ea1189-3 (pBlueSK.acrD) Ea1189-3 (pBlueKS.acrD) Ea1189-3 (pBlueKS.acrD-ext)
    Plac < < acrD Plac> > acrD Plac, PacrD> > acrD
Antimicrobials      
Benzalkonium chloride 12.5 12.5 1.2 1.2 ND
Chloramphenicol 3.1 ND 1.2 1.2 1.2
Clotrimazole > 1000 > 1000 6.2 12.5 25
Fusaric acid 500 500 500 500 500
Fusidic acid 250 250 3.1 6.2 25
Genistein > 5000 > 5000 62.5 62.5 62.5
Josamycin 125 125 3.1 3.1 3.1
Luteolin > 5000 > 5000 15.63 15.6 125
Naladixic acid 2.5 2.5 1.2 1.2 1.2
Naringenin 5000 5000 312 312 312
Nitrofurantoin 25 12.5 12.5 12.5 12.5
Norfloxacin 0.63 0.63 0.03 0.03 0.03
Novobiocin 250 250 6.2 25 100
Phloretin 5000 5000 625 625 625
Rifampicin 12.5 12.5 12.5 12.5 12.5
Tetracycline 1.5 1.5 1.2 1.2 1.2
Aminoglycosides      
Amikacin 2.5 2.5 2.5 2.5 2.5
Gentamicin 2.5 2.5 2.5 2.5 2.5
Hygromycin B 100 100 62.5 125 125
Streptomycin 2.5 2.5 2.5 2.5 2.5
Tobramycin 2.5 2.5 2.5 2.5 2.5
Macrolids      
Azithromycin 0.31 0.31 0.63 0.63 0.63
Clarithromycin 0.31 0.31 0.31 0.31 0.31
Erythromycin 0.63 0.31 0.16 0.16 0.16
Roxithromycin 1.25 1.25 0.16 0.16 0.16
Heavy metals      
Cadmium acetate 12.5 12.5 25 50 50
Cobalt (II) chloride 625 625 1250 1250 1250
Copper (II) sulfate 1250 1250 1250 1250 1250
Nickel (II) chloride 1250 1250 2500 2500 2500
Silver nitrate 12.5 6.2 6.2 6.2 6.2
Sodium tungstate 125000 62500 125000 125000 125000
Zinc sulfate 156 156 156 312 312
Dyes      
Acriflavine 50 50 6.2 6.2 6.2
Crystal violet 3.1 3.1 2.5 2.5 2.5
Ethidium bromide 250 250 3.1 3.1 6.2
Rhodamine 6G > 100 > 100 3.1 3.1 3.1
Detergents      
Bile salt 5000 5000 625 1250 5000
Deoxycholate > 1000 > 1000 312 1250 2500
SDS > 1000 > 1000 62.5 125 125
  1. aMIC values were determined by the 2-fold dilution assay in three or more independent experiments with similar results. Boldface numbers indicate a higher or lower MIC. ND, not determined.