Figure 4

Electrophoretic mobility shift analysis of BaeR interaction with Cy5-labeled DNA fragments. DNA fragments contain the promoter regions of (A) acrD (246 bp), (B) acrA (205 bp) and (C) tolC (291 bp), respectively. Approximately 0.16 pmol of the DNA fragments were incubated with increasing amounts of His-tag purified BaeR (indicated at the top of each lane). The DNA-protein complexes were separated on 4% non-denaturing polyacrylamide gels.