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Table 7 Lower limit dynamic range for MLST and subtyping MAMA primer sets

From: Real-time PCR assays for genotyping of Cryptococcus gattii in North America

Primer set tested Limit (pg) Median Ct
VGI 0.5 31.7
non-VGI 0.5 31.1
VGII 0.5 29.5
non-VGII 0.5 28.7
VGIII 0.5 28.5
non-VGIII 0.5 29.9
VGIV 0.5 33.7
non-VGIV 0.5 33.2
VGIIa 0.5 30.2
non-VGIIa 0.5 31.2
VGIIb 0.5 30.1
non-VGIIb 0.5 28.5
VGIIc 0.5 37.4
non-VGIIc 0.05 39.4