Table 5 VGII subtyping SYBR MAMA Ct values and genotype assignments for VGIIa,b,c
From: Real-time PCR assays for genotyping of Cryptococcus gattii in North America
| Â | VGIIa_Assay_45211 | VGIIb_Assay_502129 | VGIIc_Assay_257655 | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Isolate ID | Strain type via MLST | VGIIa Ct Mean | non-VGIIa Ct Mean | Delta Ct | Type call via assay | VGIIb Ct Mean | non-VGIIb Ct Mean | Delta Ct | Type call via assay | VGIIc Ct Mean | non-VGIIc Ct Mean | Delta Ct | Type call via assay | Final Call |
B6864 | VGIIa | 17.2 | 30.5 | 13.3 | VGIIa | 31.0 | 17.5 | −13.5 | non-VGIIb | 40.0 | 27.8 | −12.2 | non-VGIIc | VGIIa |
B7395 | VGIIa | 19.8 | 33.5 | 13.7 | VGIIa | 33.1 | 20.3 | −12.9 | non-VGIIb | 40.0 | 30.6 | −9.4 | non-VGIIc | VGIIa |
B7422 | VGIIa | 18.3 | 33.6 | 15.4 | VGIIa | 26.4 | 17.6 | −8.8 | non-VGIIb | 39.2 | 28.6 | −10.6 | non-VGIIc | VGIIa |
B7436 | VGIIa | 18.6 | 31.7 | 13.1 | VGIIa | 30.1 | 17.0 | −13.2 | non-VGIIb | 38.0 | 29.1 | −8.9 | non-VGIIc | VGIIa |
B7467 | VGIIa | 20.5 | 37.3 | 16.8 | VGIIa | 35.1 | 20.3 | −14.7 | non-VGIIb | 40.0 | 30.9 | −9.1 | non-VGIIc | VGIIa |
B8555 | VGIIa | 17.1 | 31.2 | 14.1 | VGIIa | 30.3 | 17.5 | −12.8 | non-VGIIb | 40.0 | 27.7 | −12.3 | non-VGIIc | VGIIa |
B8577 | VGIIa | 20.8 | 36.8 | 16.0 | VGIIa | 32.8 | 20.8 | −12.1 | non-VGIIb | 40.0 | 31.4 | −8.6 | non-VGIIc | VGIIa |
B8793 | VGIIa | 15.1 | 29.8 | 14.7 | VGIIa | 30.7 | 18.6 | −12.1 | non-VGIIb | 40.0 | 29.8 | −10.2 | non-VGIIc | VGIIa |
B8849 | VGIIa | 19.8 | 34.4 | 14.6 | VGIIa | 33.6 | 20.2 | −13.4 | non-VGIIb | 40.0 | 30.6 | −9.4 | non-VGIIc | VGIIa |
CA-1014 | VGIIa | 13.1 | 27.3 | 14.2 | VGIIa | 27.0 | 14.0 | −13.0 | non-VGIIb | 34.9 | 24.2 | −10.7 | non-VGIIc | VGIIa |
CBS-7750 | VGIIa | 21.8 | 32.2 | 10.4 | VGIIa | 33.4 | 21.5 | −11.9 | non-VGIIb | 40.0 | 34.1 | −5.9 | non-VGIIc | VGIIa |
ICB-107 | VGIIa | 21.8 | 33.6 | 11.8 | VGIIa | 33.2 | 21.2 | −12.0 | non-VGIIb | 40.0 | 33.8 | −6.2 | non-VGIIc | VGIIa |
NIH-444 | VGIIa | 14.8 | 27.3 | 12.5 | VGIIa | 28.5 | 15.3 | −13.1 | non-VGIIb | 36.1 | 25.7 | −10.3 | non-VGIIc | VGIIa |
B8508 | VGIIa | 17.0 | 27.8 | 10.8 | VGIIa | 26.5 | 17.3 | −9.2 | non-VGIIb | 31.7 | 22.7 | −9.1 | non-VGIIc | VGIIa |
B8512 | VGIIa | 17.6 | 28.1 | 10.4 | VGIIa | 26.3 | 18.0 | −8.3 | non-VGIIb | 33.2 | 24.2 | −9.0 | non-VGIIc | VGIIa |
B8558 | VGIIa | 16.3 | 24.8 | 8.5 | VGIIa | 27.3 | 15.3 | −12.0 | non-VGIIb | 29.4 | 20.0 | −9.4 | non-VGIIc | VGIIa |
B8561 | VGIIa | 15.8 | 27.5 | 11.8 | VGIIa | 25.0 | 16.9 | −8.1 | non-VGIIb | 33.4 | 23.2 | −10.2 | non-VGIIc | VGIIa |
B8563 | VGIIa | 14.5 | 27.3 | 12.8 | VGIIa | 23.9 | 15.6 | −8.3 | non-VGIIb | 31.7 | 21.7 | −10.0 | non-VGIIc | VGIIa |
B8567 | VGIIa | 15.0 | 36.2 | 21.2 | VGIIa | 24.5 | 16.0 | −8.5 | non-VGIIb | 31.8 | 22.2 | −9.5 | non-VGIIc | VGIIa |
B8854 | VGIIa | 14.7 | 26.7 | 12.0 | VGIIa | 24.1 | 15.1 | −9.0 | non-VGIIb | 31.4 | 22.2 | −9.2 | non-VGIIc | VGIIa |
B8889 | VGIIa | 17.0 | 28.1 | 11.0 | VGIIa | 25.9 | 17.3 | −8.7 | non-VGIIb | 33.2 | 23.8 | −9.4 | non-VGIIc | VGIIa |
B9077 | VGIIa | 16.7 | 27.8 | 11.1 | VGIIa | 25.6 | 16.7 | −9.0 | non-VGIIb | 32.9 | 24.4 | −8.4 | non-VGIIc | VGIIa |
B9296 | VGIIa | 17.0 | 27.5 | 10.5 | VGIIa | 25.5 | 17.3 | −8.2 | non-VGIIb | 32.9 | 24.8 | −8.1 | non-VGIIc | VGIIa |
B7394 | VGIIb | 40.0 | 19.0 | −21.0 | non-VGIIa | 17.3 | 29.6 | 12.3 | VGIIb | 40.0 | 29.0 | −11.0 | non-VGIIc | VGIIb |
B7735 | VGIIb | 31.0 | 18.3 | −12.8 | non-VGIIa | 18.7 | 31.3 | 12.6 | VGIIb | 38.1 | 28.9 | −9.3 | non-VGIIc | VGIIb |
B8554 | VGIIb | 32.9 | 21.2 | −11.7 | non-VGIIa | 22.2 | 35.0 | 12.8 | VGIIb | 40.0 | 30.4 | −9.6 | non-VGIIc | VGIIb |
B8828 | VGIIb | 31.9 | 21.1 | −10.8 | non-VGIIa | 19.9 | 35.1 | 15.2 | VGIIb | 40.0 | 30.5 | −9.5 | non-VGIIc | VGIIb |
B8211 | VGIIb | 27.8 | 16.9 | −10.9 | non-VGIIa | 17.4 | 28.8 | 11.4 | VGIIb | 32.3 | 22.3 | −10.0 | non-VGIIc | VGIIb |
B8966 | VGIIb | 26.2 | 14.7 | −11.5 | non-VGIIa | 16.3 | 24.1 | 7.9 | VGIIb | 31.8 | 23.2 | −8.6 | non-VGIIc | VGIIb |
B9076 | VGIIb | 30.0 | 18.8 | −11.2 | non-VGIIa | 19.7 | 30.9 | 11.4 | VGIIb | 39.1 | 27.0 | −12.1 | non-VGIIc | VGIIb |
B9157 | VGIIb | 29.1 | 16.6 | −12.4 | non-VGIIa | 15.4 | 23.8 | 8.5 | VGIIb | 30.3 | 21.3 | −9.0 | non-VGIIc | VGIIb |
B9170 | VGIIb | 26.6 | 15.4 | −11.2 | non-VGIIa | 16.9 | 24.8 | 7.9 | VGIIb | 31.0 | 22.7 | −8.3 | non-VGIIc | VGIIb |
B9234 | VGIIb | 26.1 | 13.9 | −12.2 | non-VGIIa | 15.3 | 23.8 | 8.5 | VGIIb | 30.2 | 21.2 | −9.1 | non-VGIIc | VGIIb |
B9290 | VGIIb | 26.1 | 13.8 | −12.3 | non-VGIIa | 15.1 | 24.5 | 9.5 | VGIIb | 30.6 | 21.2 | −9.5 | non-VGIIc | VGIIb |
B9241 | VGIIb | 26.7 | 20.2 | −6.5 | non-VGIIa | 14.5 | 24.0 | 9.4 | VGIIb | 30.5 | 21.4 | −9.1 | non-VGIIc | VGIIb |
B9428 | VGIIb | 27.5 | 14.8 | −12.6 | non-VGIIa | 16.0 | 24.3 | 8.2 | VGIIb | 32.0 | 22.4 | −9.6 | non-VGIIc | VGIIb |
B6863 | VGIIc | 31.9 | 20.3 | −11.5 | non-VGIIa | 33.4 | 20.2 | −13.2 | non-VGIIb | 27.5 | 40.0 | 12.5 | VGIIc | VGIIc |
B7390 | VGIIc | 32.7 | 18.9 | −13.8 | non-VGIIa | 31.1 | 17.9 | −13.2 | non-VGIIb | 25.9 | 40.0 | 14.1 | VGIIc | VGIIc |
B7432 | VGIIc | 40.0 | 18.5 | −21.5 | non-VGIIa | 30.7 | 17.6 | −13.1 | non-VGIIb | 25.7 | 40.0 | 14.3 | VGIIc | VGIIc |
B7434 | VGIIc | 27.5 | 15.5 | −12.0 | non-VGIIa | 28.5 | 15.4 | −13.1 | non-VGIIb | 23.3 | 40.0 | 16.7 | VGIIc | VGIIc |
B7466 | VGIIc | 31.7 | 20.8 | −10.9 | non-VGIIa | 33.5 | 20.6 | −12.8 | non-VGIIb | 28.1 | 40.0 | 11.9 | VGIIc | VGIIc |
B7491 | VGIIc | 28.7 | 17.4 | −11.2 | non-VGIIa | 30.4 | 16.9 | −13.5 | non-VGIIb | 24.0 | 40.0 | 16.0 | VGIIc | VGIIc |
B7493 | VGIIc | 28.8 | 18.3 | −10.6 | non-VGIIa | 31.1 | 18.0 | −13.1 | non-VGIIb | 25.5 | 40.0 | 14.5 | VGIIc | VGIIc |
B7641 | VGIIc | 29.2 | 17.2 | −12.0 | non-VGIIa | 30.0 | 17.2 | −12.8 | non-VGIIb | 24.5 | 40.0 | 15.5 | VGIIc | VGIIc |
B7737 | VGIIc | 32.6 | 20.1 | −12.5 | non-VGIIa | 30.8 | 20.5 | −10.4 | non-VGIIb | 28.4 | 40.0 | 11.6 | VGIIc | VGIIc |
B7765 | VGIIc | 32.2 | 19.3 | −12.8 | non-VGIIa | 32.3 | 18.9 | −13.3 | non-VGIIb | 27.5 | 40.0 | 12.5 | VGIIc | VGIIc |
B8210 | VGIIc | 29.7 | 17.6 | −12.0 | non-VGIIa | 30.1 | 17.4 | −12.7 | non-VGIIb | 25.9 | 40.0 | 14.1 | VGIIc | VGIIc |
B8214 | VGIIc | 30.1 | 17.5 | −12.5 | non-VGIIa | 30.9 | 17.5 | −13.4 | non-VGIIb | 26.1 | 40.0 | 13.9 | VGIIc | VGIIc |
B8510 | VGIIc | 29.6 | 17.5 | −12.0 | non-VGIIa | 31.0 | 17.3 | −13.7 | non-VGIIb | 24.5 | 40.0 | 15.5 | VGIIc | VGIIc |
B8549 | VGIIc | 29.9 | 17.7 | −12.1 | non-VGIIa | 31.0 | 17.8 | −13.2 | non-VGIIb | 24.8 | 40.0 | 15.2 | VGIIc | VGIIc |
B8552 | VGIIc | 29.2 | 17.1 | −12.0 | non-VGIIa | 30.3 | 17.2 | −13.1 | non-VGIIb | 24.4 | 40.0 | 15.6 | VGIIc | VGIIc |
B8571 | VGIIc | 33.0 | 20.3 | −12.7 | non-VGIIa | 32.6 | 20.2 | −12.5 | non-VGIIb | 28.1 | 40.0 | 11.9 | VGIIc | VGIIc |
B8788 | VGIIc | 29.1 | 17.3 | −11.7 | non-VGIIa | 30.0 | 17.2 | −12.8 | non-VGIIb | 25.0 | 40.0 | 15.0 | VGIIc | VGIIc |
B8798 | VGIIc | 36.5 | 22.8 | −13.7 | non-VGIIa | 34.5 | 22.2 | −12.3 | non-VGIIb | 31.0 | 40.0 | 9.0 | VGIIc | VGIIc |
B8821 | VGIIc | 37.7 | 24.5 | −13.2 | non-VGIIa | 37.1 | 24.4 | −12.7 | non-VGIIb | 33.0 | 40.0 | 7.0 | VGIIc | VGIIc |
B8825 | VGIIc | 29.6 | 17.7 | −11.9 | non-VGIIa | 30.6 | 17.7 | −12.9 | non-VGIIb | 25.8 | 40.0 | 14.2 | VGIIc | VGIIc |
B8833 | VGIIc | 29.0 | 17.0 | −12.0 | non-VGIIa | 30.1 | 17.0 | −13.1 | non-VGIIb | 25.2 | 40.0 | 14.8 | VGIIc | VGIIc |
B8838 | VGIIc | 32.0 | 19.5 | −12.5 | non-VGIIa | 32.9 | 19.3 | −13.7 | non-VGIIb | 28.7 | 40.0 | 11.3 | VGIIc | VGIIc |
B8843 | VGIIc | 32.4 | 19.9 | −12.5 | non-VGIIa | 33.0 | 19.5 | −13.5 | non-VGIIb | 28.6 | 40.0 | 11.4 | VGIIc | VGIIc |
B8853 | VGIIc | 32.8 | 21.5 | −11.3 | non-VGIIa | 36.0 | 23.4 | −12.6 | non-VGIIb | 33.1 | 40.0 | 6.9 | VGIIc | VGIIc |
B9159 | VGIIc | 27.4 | 20.3 | −7.1 | non-VGIIa | 25.8 | 16.7 | −9.1 | non-VGIIb | 20.5 | 34.5 | 14.0 | VGIIc | VGIIc |
B9227 | VGIIc | 25.6 | 13.6 | −12.0 | non-VGIIa | 23.9 | 14.9 | −9.0 | non-VGIIb | 18.0 | 31.5 | 13.4 | VGIIc | VGIIc |
B9235 | VGIIc | 25.9 | 13.7 | −12.1 | non-VGIIa | 24.1 | 14.9 | −9.2 | non-VGIIb | 18.4 | 32.4 | 14.0 | VGIIc | VGIIc |
B9244 | VGIIc | 27.2 | 19.1 | −8.1 | non-VGIIa | 26.2 | 16.9 | −9.2 | non-VGIIb | 20.2 | 32.5 | 12.3 | VGIIc | VGIIc |
B9245 | VGIIc | 28.4 | 22.9 | −5.5 | non-VGIIa | 25.2 | 17.4 | −7.8 | non-VGIIb | 20.7 | 34.5 | 13.8 | VGIIc | VGIIc |
B9295 | VGIIc | 21.0 | 17.1 | −3.8 | non-VGIIa | 26.0 | 19.6 | −6.4 | non-VGIIb | 22.1 | 28.1 | 5.9 | VGIIc | VGIIc |
B9302 | VGIIc | 26.7 | 15.6 | −11.1 | non-VGIIa | 23.7 | 15.4 | −8.3 | non-VGIIb | 19.4 | 34.3 | 15.0 | VGIIc | VGIIc |
B9374 | VGIIc | 27.4 | 21.6 | −5.8 | non-VGIIa | 24.0 | 15.3 | −8.7 | non-VGIIb | 19.4 | 33.4 | 14.0 | VGIIc | VGIIc |