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Table 4 MLST SYBR MAMA Ct values and genotype assignments for VGI-VGIV

From: Real-time PCR assays for genotyping of Cryptococcus gattii in North America

 

VGI_MPD471

VGII_MPD495

VGIII_MPD198

VGIV_MPD423

Isolate ID

Strain type via MLST

VGI Ct Mean

non-VGI Ct Mean

Delta Ct

Type call via assay

VGII Ct Mean

non-VGII Ct Mean

Delta Ct

Type call via assay

VGIII Ct Mean

non-VGIII Ct Mean

Delta Ct

Type call via assay

VGIV Ct Mean

non-VGIV Ct Mean

Delta Ct

Type call via assay

Final Call

B7488

VGI

17.0

29.0

11.9

VGI

37.4

17.7

−19.7

non-VGII

28.4

14.9

−13.5

non-VGIII

32.4

16.3

−16.1

non-VGIV

VGI

B7496

VGI

18.2

28.0

9.8

VGI

35.3

19.0

−16.3

non-VGII

24.5

16.4

−8.1

non-VGIII

31.7

17.9

−13.8

non-VGIV

VGI

B8551

VGI

17.3

29.6

12.3

VGI

36.2

17.9

−18.3

non-VGII

28.7

15.3

−13.4

non-VGIII

39.0

16.7

−22.3

non-VGIV

VGI

B8852

VGI

21.1

30.9

9.8

VGI

36.5

21.9

−14.6

non-VGII

27.8

19.1

−8.8

non-VGIII

32.0

20.6

−11.4

non-VGIV

VGI

B8886

VGI

18.9

29.2

10.3

VGI

38.1

19.3

−18.8

non-VGII

26.7

16.4

−10.3

non-VGIII

32.3

17.9

−14.4

non-VGIV

VGI

B8887

VGI

15.9

28.3

12.4

VGI

23.6

15.5

−8.1

non-VGII

33.6

16.2

−17.4

non-VGIII

34.1

15.5

−18.7

non-VGIV

VGI

B8990

VGI

18.8

30.9

12.1

VGI

37.2

20.1

−17.1

non-VGII

31.3

16.9

−14.3

non-VGIII

40.0

19.3

−20.7

non-VGIV

VGI

B9009

VGI

21.6

31.0

9.4

VGI

36.5

23.1

−13.4

non-VGII

28.6

19.4

−9.2

non-VGIII

40.0

21.1

−18.9

non-VGIV

VGI

B4501

VGI

16.1

26.7

10.6

VGI

30.5

18.1

−12.4

non-VGII

30.6

17.3

−13.3

non-VGIII

29.4

16.4

−13.0

non-VGIV

VGI

B4503

VGI

15.9

27.2

11.2

VGI

32.7

18.6

−14.1

non-VGII

33.8

17.9

−15.9

non-VGIII

28.7

16.1

−12.6

non-VGIV

VGI

B4504

VGI

15.6

27.2

11.5

VGI

33.1

18.1

−15.1

non-VGII

33.9

17.4

−16.4

non-VGIII

28.7

15.8

−13.0

non-VGIV

VGI

B4516

VGI

15.3

26.8

11.5

VGI

31.5

17.6

−13.9

non-VGII

33.4

16.8

−16.6

non-VGIII

29.7

15.3

−14.3

non-VGIV

VGI

B5765

VGI

17.2

28.0

10.8

VGI

32.8

19.7

−13.0

non-VGII

34.4

19.2

−15.2

non-VGIII

29.0

16.3

−12.7

non-VGIV

VGI

B9018

VGI

17.7

30.0

12.3

VGI

34.6

17.9

−16.7

non-VGII

31.8

18.6

−13.2

non-VGIII

35.0

18.3

−16.8

non-VGIV

VGI

B9019

VGI

16.9

26.1

9.2

VGI

35.4

16.7

−18.7

non-VGII

34.9

16.7

−18.2

non-VGIII

30.5

16.8

−13.7

non-VGIV

VGI

B9021

VGI

21.4

32.9

11.5

VGI

33.4

19.9

−13.5

non-VGII

32.7

20.5

−12.2

non-VGIII

35.5

20.4

−15.2

non-VGIV

VGI

B9142

VGI

16.0

26.3

10.3

VGI

27.8

15.9

−11.9

non-VGII

32.7

16.5

−16.2

non-VGIII

31.7

16.6

−15.1

non-VGIV

VGI

B9149

VGI

17.7

26.8

9.1

VGI

28.5

17.5

−11.0

non-VGII

28.5

18.2

−10.3

non-VGIII

31.0

18.3

−12.6

non-VGIV

VGI

B6864

VGIIa

27.8

17.5

−10.3

non-VGI

19.3

33.1

13.8

VGII

34.7

19.7

−15.0

non-VGIII

40.0

16.1

−23.9

non-VGIV

VGII

B7395

VGIIa

28.9

18.8

−10.1

non-VGI

21.3

32.6

11.3

VGII

40.0

19.2

19.2

non-VGIII

40.0

18.8

−21.2

non-VGIV

VGII

B7422

VGIIa

27.4

17.4

−10.0

non-VGI

19.5

32.3

12.8

VGII

35.4

19.1

−16.3

non-VGIII

40.0

15.6

−24.4

non-VGIV

VGII

B7436

VGIIa

27.8

17.9

−9.9

non-VGI

20.7

35.4

14.7

VGII

36.5

16.9

−19.6

non-VGIII

40.0

15.6

−24.4

non-VGIV

VGII

B7467

VGIIa

30.9

20.7

−10.1

non-VGI

22.7

32.7

9.9

VGII

37.7

23.4

−14.2

non-VGIII

40.0

19.1

−20.9

non-VGIV

VGII

B8555

VGIIa

27.9

17.7

−10.2

non-VGI

19.7

32.1

12.4

VGII

34.6

20.8

−13.8

non-VGIII

40.0

16.6

−23.4

non-VGIV

VGII

B8577

VGIIa

31.1

20.9

−10.2

non-VGI

21.8

34.1

12.3

VGII

33.1

23.4

−9.8

non-VGIII

40.0

19.8

−20.2

non-VGIV

VGII

B8793

VGIIa

27.4

17.4

−10.0

non-VGI

18.9

32.6

13.7

VGII

39.0

24.9

−14.1

non-VGIII

40.0

16.3

−23.7

non-VGIV

VGII

B8849

VGIIa

28.9

18.7

−10.1

non-VGI

22.9

35.1

12.2

VGII

36.0

22.7

−13.3

non-VGIII

40.0

18.4

−21.6

non-VGIV

VGII

CA-1014

VGIIa

20.4

11.6

−8.8

non-VGI

13.6

32.4

18.9

VGII

31.1

12.8

−18.3

non-VGIII

40.0

11.0

−29.0

non-VGIV

VGII

CBS-7750

VGIIa

27.2

17.3

−9.9

non-VGI

18.8

33.1

14.3

VGII

38.0

25.5

−12.5

non-VGIII

40.0

15.8

−24.2

non-VGIV

VGII

ICB-107

VGIIa

28.1

18.2

−9.9

non-VGI

20.0

34.7

14.8

VGII

37.5

25.4

−12.1

non-VGIII

40.0

15.6

−24.4

non-VGIV

VGII

NIH-444

VGIIa

24.9

14.9

−10.0

non-VGI

17.0

33.2

16.2

VGII

34.9

17.7

−17.2

non-VGIII

40.0

13.3

−26.7

non-VGIV

VGII

B8508

VGIIa

23.7

14.8

−8.9

non-VGI

17.4

30.4

13.0

VGII

34.5

16.2

−18.2

non-VGIII

29.1

14.9

−14.2

non-VGIV

VGII

B8512

VGIIa

23.5

14.6

−9.0

non-VGI

16.7

30.6

13.9

VGII

31.4

15.7

−15.6

non-VGIII

29.7

14.8

−14.9

non-VGIV

VGII

B8558

VGIIa

22.5

13.7

−8.8

non-VGI

15.9

29.9

14.0

VGII

30.6

14.9

−15.7

non-VGIII

30.1

14.3

−15.9

non-VGIV

VGII

B8561

VGIIa

26.5

17.7

−8.8

non-VGI

20.3

34.2

14.0

VGII

34.1

19.1

−15.0

non-VGIII

33.2

22.2

−11.0

non-VGIV

VGII

B8563

VGIIa

24.4

16.0

−8.4

non-VGI

18.4

32.8

14.4

VGII

32.8

20.4

−12.4

non-VGIII

32.2

17.3

−14.9

non-VGIV

VGII

B8567

VGIIa

25.6

17.0

−8.6

non-VGI

19.4

34.1

14.7

VGII

33.8

18.2

−15.6

non-VGIII

35.1

16.8

−18.2

non-VGIV

VGII

B8854

VGIIa

24.7

15.8

−8.9

non-VGI

18.1

32.7

14.6

VGII

33.0

17.1

−15.9

non-VGIII

33.2

15.8

−17.4

non-VGIV

VGII

B8889

VGIIa

28.0

17.6

−10.4

non-VGI

20.3

33.1

12.7

VGII

33.7

19.1

−14.6

non-VGIII

32.4

17.5

−15.0

non-VGIV

VGII

B9077

VGIIa

33.6

17.8

−15.9

non-VGI

15.4

28.6

13.2

VGII

40.0

18.6

−21.5

non-VGIII

40.0

18.6

−21.4

non-VGIV

VGII

B9296

VGIIa

27.3

19.8

−7.5

non-VGI

18.6

34.0

15.4

VGII

32.4

20.8

−11.6

non-VGIII

34.9

19.2

−15.7

non-VGIV

VGII

B7394

VGIIb

31.9

22.5

−9.5

non-VGI

23.5

33.5

10.0

VGII

33.7

19.3

−14.4

non-VGIII

40.0

20.2

−19.8

non-VGIV

VGII

B7735

VGIIb

26.9

17.8

−9.1

non-VGI

18.3

33.3

15.0

VGII

0.0

15.8

15.8

non-VGIII

40.0

15.4

−24.6

non-VGIV

VGII

B8554

VGIIb

28.8

18.3

−10.5

non-VGI

20.8

32.2

11.3

VGII

35.5

22.0

−13.4

non-VGIII

40.0

18.3

−21.7

non-VGIV

VGII

B8828

VGIIb

28.8

18.5

−10.3

non-VGI

20.7

32.7

11.9

VGII

35.9

19.2

−16.7

non-VGIII

40.0

31.9

−8.1

non-VGIV

VGII

B8211

VGIIb

22.9

12.8

−10.1

non-VGI

15.1

30.1

15.1

VGII

33.0

13.9

−19.0

non-VGIII

33.8

12.9

−21.0

non-VGIV

VGII

B8966

VGIIb

24.6

15.5

−9.0

non-VGI

17.3

25.9

8.6

VGII

29.3

15.6

−13.7

non-VGIII

28.9

14.7

−14.2

non-VGIV

VGII

B9076

VGIIb

40.0

17.5

−22.5

non-VGI

17.1

27.5

10.5

VGII

40.0

18.4

−21.6

non-VGIII

30.6

18.0

−12.6

non-VGIV

VGII

B9157

VGIIb

25.4

15.3

−10.2

non-VGI

17.6

29.4

11.9

VGII

31.2

16.1

−15.1

non-VGIII

31.6

16.1

−15.5

non-VGIV

VGII

B9170

VGIIb

26.2

16.9

−9.3

non-VGI

17.5

28.7

11.2

VGII

29.5

17.6

−11.9

non-VGIII

31.1

17.7

−13.4

non-VGIV

VGII

B9234

VGIIb

24.7

15.0

−9.6

non-VGI

15.4

30.3

14.9

VGII

30.2

15.7

−14.5

non-VGIII

33.3

15.8

−17.5

non-VGIV

VGII

B9290

VGIIb

24.8

16.0

−8.8

non-VGI

15.9

34.1

18.2

VGII

30.6

20.8

−9.7

non-VGIII

33.2

16.6

−16.6

non-VGIV

VGII

B9241

VGIIb

23.4

13.2

−10.3

non-VGI

15.5

28.0

12.5

VGII

30.0

13.9

−16.0

non-VGIII

34.0

13.5

−20.5

non-VGIV

VGII

B9428

VGIIb

25.2

14.4

−10.7

non-VGI

18.7

28.3

9.6

VGII

30.2

15.5

−14.7

non-VGIII

34.1

15.0

−19.1

non-VGIV

VGII

B6863

VGIIc

28.9

18.6

−10.2

non-VGI

20.7

34.2

13.5

VGII

33.2

22.7

−10.6

non-VGIII

40.0

18.1

−21.9

non-VGIV

VGII

B7390

VGIIc

27.7

18.3

−9.5

non-VGI

19.9

33.9

13.9

VGII

39.5

24.7

−14.8

non-VGIII

40.0

16.9

−23.1

non-VGIV

VGII

B7432

VGIIc

28.2

18.3

−9.9

non-VGI

20.0

32.6

12.7

VGII

34.8

18.0

−16.8

non-VGIII

40.0

17.2

−22.8

non-VGIV

VGII

B7434

VGIIc

25.6

16.2

−9.4

non-VGI

17.7

34.5

16.8

VGII

34.4

17.9

−16.5

non-VGIII

40.0

13.8

−26.2

non-VGIV

VGII

B7466

VGIIc

30.8

20.8

−10.0

non-VGI

22.4

33.6

11.2

VGII

37.4

23.7

−13.7

non-VGIII

40.0

19.5

−20.5

non-VGIV

VGII

B7491

VGIIc

26.9

17.3

−9.6

non-VGI

19.2

33.0

13.8

VGII

0.0

16.8

16.8

non-VGIII

40.0

16.7

−23.3

non-VGIV

VGII

B7493

VGIIc

27.1

17.4

−9.7

non-VGI

18.6

33.6

15.1

VGII

36.6

20.7

−15.8

non-VGIII

40.0

16.1

−23.9

non-VGIV

VGII

B7641

VGIIc

26.0

17.3

−8.7

non-VGI

18.7

32.3

13.7

VGII

34.3

20.0

−14.3

non-VGIII

40.0

15.6

−24.4

non-VGIV

VGII

B7737

VGIIc

28.0

18.5

−9.6

non-VGI

20.1

34.3

14.2

VGII

37.0

23.0

−14.0

non-VGIII

40.0

18.0

−22.0

non-VGIV

VGII

B7765

VGIIc

22.5

13.0

−9.5

non-VGI

14.5

34.1

19.6

VGII

33.1

23.4

−9.7

non-VGIII

40.0

12.9

−27.1

non-VGIV

VGII

B8210

VGIIc

27.8

18.1

−9.7

non-VGI

19.6

33.3

13.7

VGII

33.0

19.4

−13.5

non-VGIII

40.0

16.8

−23.2

non-VGIV

VGII

B8214

VGIIc

27.1

17.7

−9.5

non-VGI

19.8

34.9

15.1

VGII

34.1

20.1

−14.0

non-VGIII

40.0

16.1

−23.9

non-VGIV

VGII

B8510

VGIIc

26.8

17.6

−9.2

non-VGI

18.8

33.2

14.5

VGII

35.2

19.1

−16.1

non-VGIII

40.0

15.6

−24.4

non-VGIV

VGII

B8549

VGIIc

26.8

16.2

−10.6

non-VGI

18.7

33.5

14.8

VGII

37.4

20.5

−16.9

non-VGIII

40.0

29.6

−10.4

non-VGIV

VGII

B8552

VGIIc

27.1

17.0

−10.1

non-VGI

18.6

33.2

14.6

VGII

34.3

19.7

−14.6

non-VGIII

40.0

16.6

−23.4

non-VGIV

VGII

B8571

VGIIc

28.8

19.4

−9.4

non-VGI

21.5

33.4

11.9

VGII

34.5

22.8

−11.8

non-VGIII

40.0

19.5

−20.5

non-VGIV

VGII

B8788

VGIIc

26.0

16.0

−10.0

non-VGI

18.5

29.5

11.0

VGII

38.0

20.4

−17.6

non-VGIII

40.0

16.6

−23.4

non-VGIV

VGII

B8798

VGIIc

36.0

24.7

−11.4

non-VGI

26.5

33.3

6.8

VGII

37.2

19.2

−18.0

non-VGIII

40.0

22.5

−17.5

non-VGIV

VGII

B8821

VGIIc

30.5

20.5

−10.0

non-VGI

22.3

33.0

10.7

VGII

37.0

29.0

−8.0

non-VGIII

40.0

18.7

−21.3

non-VGIV

VGII

B8825

VGIIc

27.4

17.8

−9.6

non-VGI

19.6

33.7

14.1

VGII

36.0

20.5

−15.5

non-VGIII

40.0

17.5

−22.5

non-VGIV

VGII

B8833

VGIIc

29.2

20.7

−8.6

non-VGI

19.5

33.4

13.9

VGII

35.4

19.6

−15.8

non-VGIII

40.0

15.5

−24.5

non-VGIV

VGII

B8838

VGIIc

29.2

19.1

−10.1

non-VGI

21.5

32.8

11.3

VGII

32.9

22.3

−10.6

non-VGIII

40.0

18.5

−21.5

non-VGIV

VGII

B8843

VGIIc

29.5

19.4

−10.1

non-VGI

21.5

33.7

12.2

VGII

37.5

22.1

−15.4

non-VGIII

40.0

19.1

−20.9

non-VGIV

VGII

B8853

VGIIc

33.3

23.1

−10.2

non-VGI

24.8

33.7

8.9

VGII

34.2

27.8

−6.4

non-VGIII

40.0

21.5

−18.5

non-VGIV

VGII

B9159

VGIIc

29.6

17.5

−12.1

non-VGI

19.1

29.9

10.7

VGII

40.0

26.0

−14.0

non-VGIII

40.0

18.0

−22.0

non-VGIV

VGII

B9227

VGIIc

24.4

15.3

−9.1

non-VGI

15.5

28.1

12.6

VGII

27.9

16.1

−11.9

non-VGIII

31.0

16.3

−14.7

non-VGIV

VGII

B9235

VGIIc

24.6

15.1

−9.5

non-VGI

15.3

28.9

13.7

VGII

29.2

16.4

−12.7

non-VGIII

31.2

15.9

−15.3

non-VGIV

VGII

B9244

VGIIc

27.3

18.4

−8.9

non-VGI

18.5

31.8

13.3

VGII

28.2

21.0

−7.2

non-VGIII

30.6

18.8

−11.8

non-VGIV

VGII

B9245

VGIIc

26.8

17.9

−8.9

non-VGI

18.0

33.5

15.5

VGII

31.2

19.3

−11.9

non-VGIII

34.2

18.5

−15.6

non-VGIV

VGII

B9295

VGIIc

28.6

19.5

−9.1

non-VGI

19.9

40.0

20.1

VGII

33.6

25.5

−8.1

non-VGIII

34.4

20.3

−14.2

non-VGIV

VGII

B9302

VGIIc

24.6

14.1

−10.5

non-VGI

16.9

26.7

9.8

VGII

28.8

15.1

−13.7

non-VGIII

31.5

14.1

−17.3

non-VGIV

VGII

B9374

VGIIc

24.8

14.2

−10.6

non-VGI

18.2

27.3

9.1

VGII

29.1

15.2

−13.9

non-VGIII

32.8

14.4

−18.4

non-VGIV

VGII

B7415

VGIII

26.8

15.9

−10.9

non-VGI

35.0

17.7

−17.3

non-VGII

12.4

27.1

14.7

VGIII

30.9

15.9

−15.0

non-VGIV

VGIII

B7495

VGIII

28.1

18.0

−10.1

non-VGI

36.1

18.8

−17.3

non-VGII

14.1

30.1

16.0

VGIII

31.8

17.6

−14.2

non-VGIV

VGIII

B8212

VGIII

26.0

15.7

−10.3

non-VGI

35.3

17.0

−18.3

non-VGII

12.4

28.5

16.1

VGIII

32.5

15.6

−16.9

non-VGIV

VGIII

B8260

VGIII

29.6

19.6

−10.0

non-VGI

36.7

20.8

−15.9

non-VGII

15.9

30.7

14.8

VGIII

36.0

19.1

−16.9

non-VGIV

VGIII

B8262

VGIII

27.2

17.2

−10.0

non-VGI

33.8

18.3

−15.5

non-VGII

13.5

30.0

16.4

VGIII

40.0

16.9

−23.1

non-VGIV

VGIII

B8516/B8616

VGIII

28.4

18.5

−9.9

non-VGI

37.8

19.5

−18.3

non-VGII

14.6

29.1

14.5

VGIII

31.8

18.0

−13.8

non-VGIV

VGIII

B9143

VGIII

28.6

18.3

−10.3

non-VGI

38.3

19.6

−18.7

non-VGII

14.5

30.2

15.7

VGIII

33.3

18.0

−15.3

non-VGIV

VGIII

B9146

VGIII

30.3

19.5

−10.8

non-VGI

38.5

21.2

−17.3

non-VGII

15.8

30.1

14.3

VGIII

31.2

19.3

−11.9

non-VGIV

VGIII

B8965

VGIII

26.2

16.8

−9.4

non-VGI

30.6

17.1

−13.5

non-VGII

16.1

30.6

14.5

VGIII

35.0

17.4

−17.6

non-VGIV

VGIII

B9148

VGIII

26.0

16.6

−9.4

non-VGI

31.0

16.6

−14.4

non-VGII

15.9

30.6

14.7

VGIII

32.8

17.4

−15.4

non-VGIV

VGIII

B9151

VGIII

25.7

16.5

−9.3

non-VGI

30.7

16.2

−14.4

non-VGII

15.4

30.3

14.9

VGIII

34.9

18.0

−17.0

non-VGIV

VGIII

B9163

VGIII

26.9

17.5

−9.4

non-VGI

29.8

17.3

−12.5

non-VGII

16.9

29.7

12.8

VGIII

33.4

18.0

−15.4

non-VGIV

VGIII

B9237

VGIII

26.7

17.9

−8.9

non-VGI

31.6

17.4

−14.2

non-VGII

17.3

35.0

17.7

VGIII

38.1

19.3

−18.9

non-VGIV

VGIII

B9372

VGIII

23.5

12.7

−10.9

non-VGI

29.3

13.1

−16.1

non-VGII

14.8

27.4

12.6

VGIII

32.6

13.0

−19.6

non-VGIV

VGIII

B9422

VGIII

23.9

12.8

−11.1

non-VGI

28.9

12.9

−15.9

non-VGII

14.6

26.8

12.2

VGIII

33.0

13.3

−19.7

non-VGIV

VGIII

B9430

VGIII

23.5

12.9

−10.6

non-VGI

30.1

13.4

−16.8

non-VGII

15.1

28.5

13.4

VGIII

35.5

13.4

−22.0

non-VGIV

VGIII

B7238

VGIV

25.2

16.4

−8.8

non-VGI

33.2

18.5

−14.7

non-VGII

34.6

17.9

−16.7

non-VGIII

16.3

27.4

11.1

VGIV

VGIV

B7240

VGIV

25.8

17.1

−8.8

non-VGI

33.9

19.5

−14.5

non-VGII

34.2

18.5

−15.7

non-VGIII

17.0

28.8

11.8

VGIV

VGIV

B7243

VGIV

26.1

17.3

−8.8

non-VGI

32.0

19.6

−12.4

non-VGII

32.3

18.7

−13.6

non-VGIII

16.8

27.1

10.2

VGIV

VGIV

B7247

VGIV

25.6

16.5

−9.1

non-VGI

33.4

19.2

−14.2

non-VGII

32.0

18.1

−13.9

non-VGIII

16.3

28.4

12.1

VGIV

VGIV

B7249

VGIV

23.4

14.8

−8.6

non-VGI

31.6

16.7

−14.9

non-VGII

32.6

16.0

−16.6

non-VGIII

14.5

31.1

16.5

VGIV

VGIV

B7260

VGIV

26.0

16.5

−9.4

non-VGI

30.9

18.0

−13.0

non-VGII

34.2

17.4

−16.8

non-VGIII

15.7

27.0

11.2

VGIV

VGIV

B7262

VGIV

26.3

16.8

−9.5

non-VGI

31.4

18.7

−12.7

non-VGII

33.4

18.0

−15.4

non-VGIII

15.8

27.5

11.6

VGIV

VGIV

B7263

VGIV

24.5

15.7

−8.9

non-VGI

33.1

17.9

−15.3

non-VGII

37.3

17.0

−20.3

non-VGIII

15.8

28.0

12.2

VGIV

VGIV

B7264

VGIV

24.4

15.0

−9.4

non-VGI

31.2

16.9

−14.3

non-VGII

30.6

16.0

−14.6

non-VGIII

14.8

26.8

12.0

VGIV

VGIV

B7265

VGIV

27.5

17.3

−10.2

non-VGI

34.1

19.6

−14.5

non-VGII

32.1

18.8

−13.3

non-VGIII

16.9

28.8

11.9

VGIV

VGIV