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Table 4 MLST SYBR MAMA Ct values and genotype assignments for VGI-VGIV

From: Real-time PCR assays for genotyping of Cryptococcus gattii in North America

  VGI_MPD471 VGII_MPD495 VGIII_MPD198 VGIV_MPD423
Isolate ID Strain type via MLST VGI Ct Mean non-VGI Ct Mean Delta Ct Type call via assay VGII Ct Mean non-VGII Ct Mean Delta Ct Type call via assay VGIII Ct Mean non-VGIII Ct Mean Delta Ct Type call via assay VGIV Ct Mean non-VGIV Ct Mean Delta Ct Type call via assay Final Call
B7488 VGI 17.0 29.0 11.9 VGI 37.4 17.7 −19.7 non-VGII 28.4 14.9 −13.5 non-VGIII 32.4 16.3 −16.1 non-VGIV VGI
B7496 VGI 18.2 28.0 9.8 VGI 35.3 19.0 −16.3 non-VGII 24.5 16.4 −8.1 non-VGIII 31.7 17.9 −13.8 non-VGIV VGI
B8551 VGI 17.3 29.6 12.3 VGI 36.2 17.9 −18.3 non-VGII 28.7 15.3 −13.4 non-VGIII 39.0 16.7 −22.3 non-VGIV VGI
B8852 VGI 21.1 30.9 9.8 VGI 36.5 21.9 −14.6 non-VGII 27.8 19.1 −8.8 non-VGIII 32.0 20.6 −11.4 non-VGIV VGI
B8886 VGI 18.9 29.2 10.3 VGI 38.1 19.3 −18.8 non-VGII 26.7 16.4 −10.3 non-VGIII 32.3 17.9 −14.4 non-VGIV VGI
B8887 VGI 15.9 28.3 12.4 VGI 23.6 15.5 −8.1 non-VGII 33.6 16.2 −17.4 non-VGIII 34.1 15.5 −18.7 non-VGIV VGI
B8990 VGI 18.8 30.9 12.1 VGI 37.2 20.1 −17.1 non-VGII 31.3 16.9 −14.3 non-VGIII 40.0 19.3 −20.7 non-VGIV VGI
B9009 VGI 21.6 31.0 9.4 VGI 36.5 23.1 −13.4 non-VGII 28.6 19.4 −9.2 non-VGIII 40.0 21.1 −18.9 non-VGIV VGI
B4501 VGI 16.1 26.7 10.6 VGI 30.5 18.1 −12.4 non-VGII 30.6 17.3 −13.3 non-VGIII 29.4 16.4 −13.0 non-VGIV VGI
B4503 VGI 15.9 27.2 11.2 VGI 32.7 18.6 −14.1 non-VGII 33.8 17.9 −15.9 non-VGIII 28.7 16.1 −12.6 non-VGIV VGI
B4504 VGI 15.6 27.2 11.5 VGI 33.1 18.1 −15.1 non-VGII 33.9 17.4 −16.4 non-VGIII 28.7 15.8 −13.0 non-VGIV VGI
B4516 VGI 15.3 26.8 11.5 VGI 31.5 17.6 −13.9 non-VGII 33.4 16.8 −16.6 non-VGIII 29.7 15.3 −14.3 non-VGIV VGI
B5765 VGI 17.2 28.0 10.8 VGI 32.8 19.7 −13.0 non-VGII 34.4 19.2 −15.2 non-VGIII 29.0 16.3 −12.7 non-VGIV VGI
B9018 VGI 17.7 30.0 12.3 VGI 34.6 17.9 −16.7 non-VGII 31.8 18.6 −13.2 non-VGIII 35.0 18.3 −16.8 non-VGIV VGI
B9019 VGI 16.9 26.1 9.2 VGI 35.4 16.7 −18.7 non-VGII 34.9 16.7 −18.2 non-VGIII 30.5 16.8 −13.7 non-VGIV VGI
B9021 VGI 21.4 32.9 11.5 VGI 33.4 19.9 −13.5 non-VGII 32.7 20.5 −12.2 non-VGIII 35.5 20.4 −15.2 non-VGIV VGI
B9142 VGI 16.0 26.3 10.3 VGI 27.8 15.9 −11.9 non-VGII 32.7 16.5 −16.2 non-VGIII 31.7 16.6 −15.1 non-VGIV VGI
B9149 VGI 17.7 26.8 9.1 VGI 28.5 17.5 −11.0 non-VGII 28.5 18.2 −10.3 non-VGIII 31.0 18.3 −12.6 non-VGIV VGI
B6864 VGIIa 27.8 17.5 −10.3 non-VGI 19.3 33.1 13.8 VGII 34.7 19.7 −15.0 non-VGIII 40.0 16.1 −23.9 non-VGIV VGII
B7395 VGIIa 28.9 18.8 −10.1 non-VGI 21.3 32.6 11.3 VGII 40.0 19.2 19.2 non-VGIII 40.0 18.8 −21.2 non-VGIV VGII
B7422 VGIIa 27.4 17.4 −10.0 non-VGI 19.5 32.3 12.8 VGII 35.4 19.1 −16.3 non-VGIII 40.0 15.6 −24.4 non-VGIV VGII
B7436 VGIIa 27.8 17.9 −9.9 non-VGI 20.7 35.4 14.7 VGII 36.5 16.9 −19.6 non-VGIII 40.0 15.6 −24.4 non-VGIV VGII
B7467 VGIIa 30.9 20.7 −10.1 non-VGI 22.7 32.7 9.9 VGII 37.7 23.4 −14.2 non-VGIII 40.0 19.1 −20.9 non-VGIV VGII
B8555 VGIIa 27.9 17.7 −10.2 non-VGI 19.7 32.1 12.4 VGII 34.6 20.8 −13.8 non-VGIII 40.0 16.6 −23.4 non-VGIV VGII
B8577 VGIIa 31.1 20.9 −10.2 non-VGI 21.8 34.1 12.3 VGII 33.1 23.4 −9.8 non-VGIII 40.0 19.8 −20.2 non-VGIV VGII
B8793 VGIIa 27.4 17.4 −10.0 non-VGI 18.9 32.6 13.7 VGII 39.0 24.9 −14.1 non-VGIII 40.0 16.3 −23.7 non-VGIV VGII
B8849 VGIIa 28.9 18.7 −10.1 non-VGI 22.9 35.1 12.2 VGII 36.0 22.7 −13.3 non-VGIII 40.0 18.4 −21.6 non-VGIV VGII
CA-1014 VGIIa 20.4 11.6 −8.8 non-VGI 13.6 32.4 18.9 VGII 31.1 12.8 −18.3 non-VGIII 40.0 11.0 −29.0 non-VGIV VGII
CBS-7750 VGIIa 27.2 17.3 −9.9 non-VGI 18.8 33.1 14.3 VGII 38.0 25.5 −12.5 non-VGIII 40.0 15.8 −24.2 non-VGIV VGII
ICB-107 VGIIa 28.1 18.2 −9.9 non-VGI 20.0 34.7 14.8 VGII 37.5 25.4 −12.1 non-VGIII 40.0 15.6 −24.4 non-VGIV VGII
NIH-444 VGIIa 24.9 14.9 −10.0 non-VGI 17.0 33.2 16.2 VGII 34.9 17.7 −17.2 non-VGIII 40.0 13.3 −26.7 non-VGIV VGII
B8508 VGIIa 23.7 14.8 −8.9 non-VGI 17.4 30.4 13.0 VGII 34.5 16.2 −18.2 non-VGIII 29.1 14.9 −14.2 non-VGIV VGII
B8512 VGIIa 23.5 14.6 −9.0 non-VGI 16.7 30.6 13.9 VGII 31.4 15.7 −15.6 non-VGIII 29.7 14.8 −14.9 non-VGIV VGII
B8558 VGIIa 22.5 13.7 −8.8 non-VGI 15.9 29.9 14.0 VGII 30.6 14.9 −15.7 non-VGIII 30.1 14.3 −15.9 non-VGIV VGII
B8561 VGIIa 26.5 17.7 −8.8 non-VGI 20.3 34.2 14.0 VGII 34.1 19.1 −15.0 non-VGIII 33.2 22.2 −11.0 non-VGIV VGII
B8563 VGIIa 24.4 16.0 −8.4 non-VGI 18.4 32.8 14.4 VGII 32.8 20.4 −12.4 non-VGIII 32.2 17.3 −14.9 non-VGIV VGII
B8567 VGIIa 25.6 17.0 −8.6 non-VGI 19.4 34.1 14.7 VGII 33.8 18.2 −15.6 non-VGIII 35.1 16.8 −18.2 non-VGIV VGII
B8854 VGIIa 24.7 15.8 −8.9 non-VGI 18.1 32.7 14.6 VGII 33.0 17.1 −15.9 non-VGIII 33.2 15.8 −17.4 non-VGIV VGII
B8889 VGIIa 28.0 17.6 −10.4 non-VGI 20.3 33.1 12.7 VGII 33.7 19.1 −14.6 non-VGIII 32.4 17.5 −15.0 non-VGIV VGII
B9077 VGIIa 33.6 17.8 −15.9 non-VGI 15.4 28.6 13.2 VGII 40.0 18.6 −21.5 non-VGIII 40.0 18.6 −21.4 non-VGIV VGII
B9296 VGIIa 27.3 19.8 −7.5 non-VGI 18.6 34.0 15.4 VGII 32.4 20.8 −11.6 non-VGIII 34.9 19.2 −15.7 non-VGIV VGII
B7394 VGIIb 31.9 22.5 −9.5 non-VGI 23.5 33.5 10.0 VGII 33.7 19.3 −14.4 non-VGIII 40.0 20.2 −19.8 non-VGIV VGII
B7735 VGIIb 26.9 17.8 −9.1 non-VGI 18.3 33.3 15.0 VGII 0.0 15.8 15.8 non-VGIII 40.0 15.4 −24.6 non-VGIV VGII
B8554 VGIIb 28.8 18.3 −10.5 non-VGI 20.8 32.2 11.3 VGII 35.5 22.0 −13.4 non-VGIII 40.0 18.3 −21.7 non-VGIV VGII
B8828 VGIIb 28.8 18.5 −10.3 non-VGI 20.7 32.7 11.9 VGII 35.9 19.2 −16.7 non-VGIII 40.0 31.9 −8.1 non-VGIV VGII
B8211 VGIIb 22.9 12.8 −10.1 non-VGI 15.1 30.1 15.1 VGII 33.0 13.9 −19.0 non-VGIII 33.8 12.9 −21.0 non-VGIV VGII
B8966 VGIIb 24.6 15.5 −9.0 non-VGI 17.3 25.9 8.6 VGII 29.3 15.6 −13.7 non-VGIII 28.9 14.7 −14.2 non-VGIV VGII
B9076 VGIIb 40.0 17.5 −22.5 non-VGI 17.1 27.5 10.5 VGII 40.0 18.4 −21.6 non-VGIII 30.6 18.0 −12.6 non-VGIV VGII
B9157 VGIIb 25.4 15.3 −10.2 non-VGI 17.6 29.4 11.9 VGII 31.2 16.1 −15.1 non-VGIII 31.6 16.1 −15.5 non-VGIV VGII
B9170 VGIIb 26.2 16.9 −9.3 non-VGI 17.5 28.7 11.2 VGII 29.5 17.6 −11.9 non-VGIII 31.1 17.7 −13.4 non-VGIV VGII
B9234 VGIIb 24.7 15.0 −9.6 non-VGI 15.4 30.3 14.9 VGII 30.2 15.7 −14.5 non-VGIII 33.3 15.8 −17.5 non-VGIV VGII
B9290 VGIIb 24.8 16.0 −8.8 non-VGI 15.9 34.1 18.2 VGII 30.6 20.8 −9.7 non-VGIII 33.2 16.6 −16.6 non-VGIV VGII
B9241 VGIIb 23.4 13.2 −10.3 non-VGI 15.5 28.0 12.5 VGII 30.0 13.9 −16.0 non-VGIII 34.0 13.5 −20.5 non-VGIV VGII
B9428 VGIIb 25.2 14.4 −10.7 non-VGI 18.7 28.3 9.6 VGII 30.2 15.5 −14.7 non-VGIII 34.1 15.0 −19.1 non-VGIV VGII
B6863 VGIIc 28.9 18.6 −10.2 non-VGI 20.7 34.2 13.5 VGII 33.2 22.7 −10.6 non-VGIII 40.0 18.1 −21.9 non-VGIV VGII
B7390 VGIIc 27.7 18.3 −9.5 non-VGI 19.9 33.9 13.9 VGII 39.5 24.7 −14.8 non-VGIII 40.0 16.9 −23.1 non-VGIV VGII
B7432 VGIIc 28.2 18.3 −9.9 non-VGI 20.0 32.6 12.7 VGII 34.8 18.0 −16.8 non-VGIII 40.0 17.2 −22.8 non-VGIV VGII
B7434 VGIIc 25.6 16.2 −9.4 non-VGI 17.7 34.5 16.8 VGII 34.4 17.9 −16.5 non-VGIII 40.0 13.8 −26.2 non-VGIV VGII
B7466 VGIIc 30.8 20.8 −10.0 non-VGI 22.4 33.6 11.2 VGII 37.4 23.7 −13.7 non-VGIII 40.0 19.5 −20.5 non-VGIV VGII
B7491 VGIIc 26.9 17.3 −9.6 non-VGI 19.2 33.0 13.8 VGII 0.0 16.8 16.8 non-VGIII 40.0 16.7 −23.3 non-VGIV VGII
B7493 VGIIc 27.1 17.4 −9.7 non-VGI 18.6 33.6 15.1 VGII 36.6 20.7 −15.8 non-VGIII 40.0 16.1 −23.9 non-VGIV VGII
B7641 VGIIc 26.0 17.3 −8.7 non-VGI 18.7 32.3 13.7 VGII 34.3 20.0 −14.3 non-VGIII 40.0 15.6 −24.4 non-VGIV VGII
B7737 VGIIc 28.0 18.5 −9.6 non-VGI 20.1 34.3 14.2 VGII 37.0 23.0 −14.0 non-VGIII 40.0 18.0 −22.0 non-VGIV VGII
B7765 VGIIc 22.5 13.0 −9.5 non-VGI 14.5 34.1 19.6 VGII 33.1 23.4 −9.7 non-VGIII 40.0 12.9 −27.1 non-VGIV VGII
B8210 VGIIc 27.8 18.1 −9.7 non-VGI 19.6 33.3 13.7 VGII 33.0 19.4 −13.5 non-VGIII 40.0 16.8 −23.2 non-VGIV VGII
B8214 VGIIc 27.1 17.7 −9.5 non-VGI 19.8 34.9 15.1 VGII 34.1 20.1 −14.0 non-VGIII 40.0 16.1 −23.9 non-VGIV VGII
B8510 VGIIc 26.8 17.6 −9.2 non-VGI 18.8 33.2 14.5 VGII 35.2 19.1 −16.1 non-VGIII 40.0 15.6 −24.4 non-VGIV VGII
B8549 VGIIc 26.8 16.2 −10.6 non-VGI 18.7 33.5 14.8 VGII 37.4 20.5 −16.9 non-VGIII 40.0 29.6 −10.4 non-VGIV VGII
B8552 VGIIc 27.1 17.0 −10.1 non-VGI 18.6 33.2 14.6 VGII 34.3 19.7 −14.6 non-VGIII 40.0 16.6 −23.4 non-VGIV VGII
B8571 VGIIc 28.8 19.4 −9.4 non-VGI 21.5 33.4 11.9 VGII 34.5 22.8 −11.8 non-VGIII 40.0 19.5 −20.5 non-VGIV VGII
B8788 VGIIc 26.0 16.0 −10.0 non-VGI 18.5 29.5 11.0 VGII 38.0 20.4 −17.6 non-VGIII 40.0 16.6 −23.4 non-VGIV VGII
B8798 VGIIc 36.0 24.7 −11.4 non-VGI 26.5 33.3 6.8 VGII 37.2 19.2 −18.0 non-VGIII 40.0 22.5 −17.5 non-VGIV VGII
B8821 VGIIc 30.5 20.5 −10.0 non-VGI 22.3 33.0 10.7 VGII 37.0 29.0 −8.0 non-VGIII 40.0 18.7 −21.3 non-VGIV VGII
B8825 VGIIc 27.4 17.8 −9.6 non-VGI 19.6 33.7 14.1 VGII 36.0 20.5 −15.5 non-VGIII 40.0 17.5 −22.5 non-VGIV VGII
B8833 VGIIc 29.2 20.7 −8.6 non-VGI 19.5 33.4 13.9 VGII 35.4 19.6 −15.8 non-VGIII 40.0 15.5 −24.5 non-VGIV VGII
B8838 VGIIc 29.2 19.1 −10.1 non-VGI 21.5 32.8 11.3 VGII 32.9 22.3 −10.6 non-VGIII 40.0 18.5 −21.5 non-VGIV VGII
B8843 VGIIc 29.5 19.4 −10.1 non-VGI 21.5 33.7 12.2 VGII 37.5 22.1 −15.4 non-VGIII 40.0 19.1 −20.9 non-VGIV VGII
B8853 VGIIc 33.3 23.1 −10.2 non-VGI 24.8 33.7 8.9 VGII 34.2 27.8 −6.4 non-VGIII 40.0 21.5 −18.5 non-VGIV VGII
B9159 VGIIc 29.6 17.5 −12.1 non-VGI 19.1 29.9 10.7 VGII 40.0 26.0 −14.0 non-VGIII 40.0 18.0 −22.0 non-VGIV VGII
B9227 VGIIc 24.4 15.3 −9.1 non-VGI 15.5 28.1 12.6 VGII 27.9 16.1 −11.9 non-VGIII 31.0 16.3 −14.7 non-VGIV VGII
B9235 VGIIc 24.6 15.1 −9.5 non-VGI 15.3 28.9 13.7 VGII 29.2 16.4 −12.7 non-VGIII 31.2 15.9 −15.3 non-VGIV VGII
B9244 VGIIc 27.3 18.4 −8.9 non-VGI 18.5 31.8 13.3 VGII 28.2 21.0 −7.2 non-VGIII 30.6 18.8 −11.8 non-VGIV VGII
B9245 VGIIc 26.8 17.9 −8.9 non-VGI 18.0 33.5 15.5 VGII 31.2 19.3 −11.9 non-VGIII 34.2 18.5 −15.6 non-VGIV VGII
B9295 VGIIc 28.6 19.5 −9.1 non-VGI 19.9 40.0 20.1 VGII 33.6 25.5 −8.1 non-VGIII 34.4 20.3 −14.2 non-VGIV VGII
B9302 VGIIc 24.6 14.1 −10.5 non-VGI 16.9 26.7 9.8 VGII 28.8 15.1 −13.7 non-VGIII 31.5 14.1 −17.3 non-VGIV VGII
B9374 VGIIc 24.8 14.2 −10.6 non-VGI 18.2 27.3 9.1 VGII 29.1 15.2 −13.9 non-VGIII 32.8 14.4 −18.4 non-VGIV VGII
B7415 VGIII 26.8 15.9 −10.9 non-VGI 35.0 17.7 −17.3 non-VGII 12.4 27.1 14.7 VGIII 30.9 15.9 −15.0 non-VGIV VGIII
B7495 VGIII 28.1 18.0 −10.1 non-VGI 36.1 18.8 −17.3 non-VGII 14.1 30.1 16.0 VGIII 31.8 17.6 −14.2 non-VGIV VGIII
B8212 VGIII 26.0 15.7 −10.3 non-VGI 35.3 17.0 −18.3 non-VGII 12.4 28.5 16.1 VGIII 32.5 15.6 −16.9 non-VGIV VGIII
B8260 VGIII 29.6 19.6 −10.0 non-VGI 36.7 20.8 −15.9 non-VGII 15.9 30.7 14.8 VGIII 36.0 19.1 −16.9 non-VGIV VGIII
B8262 VGIII 27.2 17.2 −10.0 non-VGI 33.8 18.3 −15.5 non-VGII 13.5 30.0 16.4 VGIII 40.0 16.9 −23.1 non-VGIV VGIII
B8516/B8616 VGIII 28.4 18.5 −9.9 non-VGI 37.8 19.5 −18.3 non-VGII 14.6 29.1 14.5 VGIII 31.8 18.0 −13.8 non-VGIV VGIII
B9143 VGIII 28.6 18.3 −10.3 non-VGI 38.3 19.6 −18.7 non-VGII 14.5 30.2 15.7 VGIII 33.3 18.0 −15.3 non-VGIV VGIII
B9146 VGIII 30.3 19.5 −10.8 non-VGI 38.5 21.2 −17.3 non-VGII 15.8 30.1 14.3 VGIII 31.2 19.3 −11.9 non-VGIV VGIII
B8965 VGIII 26.2 16.8 −9.4 non-VGI 30.6 17.1 −13.5 non-VGII 16.1 30.6 14.5 VGIII 35.0 17.4 −17.6 non-VGIV VGIII
B9148 VGIII 26.0 16.6 −9.4 non-VGI 31.0 16.6 −14.4 non-VGII 15.9 30.6 14.7 VGIII 32.8 17.4 −15.4 non-VGIV VGIII
B9151 VGIII 25.7 16.5 −9.3 non-VGI 30.7 16.2 −14.4 non-VGII 15.4 30.3 14.9 VGIII 34.9 18.0 −17.0 non-VGIV VGIII
B9163 VGIII 26.9 17.5 −9.4 non-VGI 29.8 17.3 −12.5 non-VGII 16.9 29.7 12.8 VGIII 33.4 18.0 −15.4 non-VGIV VGIII
B9237 VGIII 26.7 17.9 −8.9 non-VGI 31.6 17.4 −14.2 non-VGII 17.3 35.0 17.7 VGIII 38.1 19.3 −18.9 non-VGIV VGIII
B9372 VGIII 23.5 12.7 −10.9 non-VGI 29.3 13.1 −16.1 non-VGII 14.8 27.4 12.6 VGIII 32.6 13.0 −19.6 non-VGIV VGIII
B9422 VGIII 23.9 12.8 −11.1 non-VGI 28.9 12.9 −15.9 non-VGII 14.6 26.8 12.2 VGIII 33.0 13.3 −19.7 non-VGIV VGIII
B9430 VGIII 23.5 12.9 −10.6 non-VGI 30.1 13.4 −16.8 non-VGII 15.1 28.5 13.4 VGIII 35.5 13.4 −22.0 non-VGIV VGIII
B7238 VGIV 25.2 16.4 −8.8 non-VGI 33.2 18.5 −14.7 non-VGII 34.6 17.9 −16.7 non-VGIII 16.3 27.4 11.1 VGIV VGIV
B7240 VGIV 25.8 17.1 −8.8 non-VGI 33.9 19.5 −14.5 non-VGII 34.2 18.5 −15.7 non-VGIII 17.0 28.8 11.8 VGIV VGIV
B7243 VGIV 26.1 17.3 −8.8 non-VGI 32.0 19.6 −12.4 non-VGII 32.3 18.7 −13.6 non-VGIII 16.8 27.1 10.2 VGIV VGIV
B7247 VGIV 25.6 16.5 −9.1 non-VGI 33.4 19.2 −14.2 non-VGII 32.0 18.1 −13.9 non-VGIII 16.3 28.4 12.1 VGIV VGIV
B7249 VGIV 23.4 14.8 −8.6 non-VGI 31.6 16.7 −14.9 non-VGII 32.6 16.0 −16.6 non-VGIII 14.5 31.1 16.5 VGIV VGIV
B7260 VGIV 26.0 16.5 −9.4 non-VGI 30.9 18.0 −13.0 non-VGII 34.2 17.4 −16.8 non-VGIII 15.7 27.0 11.2 VGIV VGIV
B7262 VGIV 26.3 16.8 −9.5 non-VGI 31.4 18.7 −12.7 non-VGII 33.4 18.0 −15.4 non-VGIII 15.8 27.5 11.6 VGIV VGIV
B7263 VGIV 24.5 15.7 −8.9 non-VGI 33.1 17.9 −15.3 non-VGII 37.3 17.0 −20.3 non-VGIII 15.8 28.0 12.2 VGIV VGIV
B7264 VGIV 24.4 15.0 −9.4 non-VGI 31.2 16.9 −14.3 non-VGII 30.6 16.0 −14.6 non-VGIII 14.8 26.8 12.0 VGIV VGIV
B7265 VGIV 27.5 17.3 −10.2 non-VGI 34.1 19.6 −14.5 non-VGII 32.1 18.8 −13.3 non-VGIII 16.9 28.8 11.9 VGIV VGIV