More than fishing in the dark: PCR of a dispersed sequence produces simple but ultrasensitive Wolbachia detection

Table 1 Number of matches to ARM in complete and draft Wolbachia genomes

Wolbachia	Supergroup	Host	Number of matches to ARM	GenBank references
w Mel	A	Drosophila melanogaster	24	NC_002978; [8]
w Ha	A	Drosophila simulans	23	CP003884; [23]
w Ri	A	Drosophila simulans	21	NC_012416; [22]
w Wil	A	Drosophila willistoni	17^a	ASM15358v1; TSC#14030-0811.24
w Ana	A	Drosophila ananassae	20^a	ASM16747v1; [24]
w Uni	A	Muscidifax uniraptor	7^a	wUni_1.0; [22]
w Suzi	A	Drosophila suzukii	23^a	CAOU02000000; [25]
w Gmm	A	Glossina morsitans morsitans	20^a	[14]
w No	B	Drosophila simulans	0^b	CP003883; [23]
w VitB	B	Nasonia vitripennis	0^b	WVB_1.0; [26]
w Pip	B	Culex quinquefasciatus	0^b	NC_010981.1; [27]
w Oo	C	Onchocerca ochengi	0^b	NC_018267.1; [28]
w Ov	C	Onchocerca volvulus	0^b	ASM33837v1; [29]
w Bm	D	Brugia malayi	0^b	NC_006833.1; [30]

Number of matches in column four refer to hits of the 315 bp ARM-PCR amplicon in the searched Wolbachia genomes. Hits were produced using the blastn algorithm (megablast) with match/mismatch scores 1,-2. Wolbachia strains are organized by supergroup (column two). Matches to ARM were only found within the A-supergroup. ^aMinimum number of ARMs in the corresponding genome. Exact number cannot be given due to the lack of a complete genome. ^bRefers to no similarity detected between ARM and searched genome (complete/draft).

ISSN: 1471-2180