Figure 2

Glycolipids in Δ cpoA and piperacillin resistant laboratory mutants containing cpoA mutations. Lipids extracted from strain R6 and from cpoA mutants, P104, P106, and R6ΔcpoA as indicated above the lanes were separated by thin layer chromatography (chloroform/methanol/acetic acid = 80:15:8). GalGalDAG (S1) and GlcDAG (S2) were used as a standards. Spots were assigned to the two major glycolipids of S. pneumoniae diglycosyl DAG (GalGlcDAG) and monoglycosyl DAG (GlcDAG).