Table 2 Bacterial strains and plasmids used in this study
Strain or plasmid | Relevant feature(s) | Source or reference | |
|---|---|---|---|
A. baumannii strains | ATCC 17978 | Wild-type strain | ATCC |
|  | AB1026 (ΔbaeR::kan r) | Derived from ATCC 17978. baeR mutant obtained by kan r gene replacement | This study |
| Â | AB1027 | AB1026 baeR::pWH1266 | This study |
| Â | AB1028 | ATCC 17978 baeR::pWH1266 | This study |
| Â | AB1029 | ATCC 17978 kan:: pWH1266 | This study |
| Â | ABtc | Induced tigecycline resistant ATCC 17978 | This study |
|  | ABtcm (ΔbaeR::kan r) | Derived from ABtc. baeR mutant obtained by kan r gene replacement | This study |
| Â | ABhl1 | Tigecycline resistant clinical isolate | This study |
E. coli strains | XL1 blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F’ proAB lacI q Z ΔM15 Tn10 (Tetr)] | Stratagene |
| Â | S17-1 (ATCC 47055) | thi pro hsdR hsdM recA[RP42-Tc::Mu- Km::Tn7 (TprSmr)Tra+] | ATCC |
Plasmids | pEX18Tc | Suicide vector containing sacB, Tcr | 40 |
| Â | pSFS2A | Containing kan r, an FRT site, FLP1, and CaSAT1 as a SAT1 flipper | 41 |
|  | pEX18Tc-Δbae::kan r | pEX18Tc containing baeR upstream and downstream fragments joined by a kan r cassette | This study |
| Â | pWH1266 (ATCC 77092) | E. coli-A. baumannii shuttle cloning vector, containing Ampr, Tetr | 43 |
| Â | pC2HP | Provided kan r for pWH1266 | 42 |
| Â | pWH1266-kan r | pWH1266 containing kan r | This study |
| Â | pWH1266-kan r-baeR | pWH1266-kan r containing baeR | This study |