Table 1 Oligonucleotides used in this study
Primer name | Sequence (5′ to 3′)a |
|---|---|
baeS-co_F | CGCGTAGTACAGGTGGAACA |
baeR-co_R | TCCACTCATGACGGTTACCA |
kan r-Bam HI_F | ATATGGATCC CCGGAATTGCCAGCTGGGGC |
kan r-Kpn I_R | ATATGGTACC TCAGAAGAACTCGTCAAGAA |
baeR-up-Sal I_F | TTAAGTCGAC CGCCCGATTATGGTCAATAG |
baeR-up-Bam HI_R | TATAGGATCC GCTTACTTCGAACCCAGCAG |
baeR-dw-Kpn I_F | ATCGGGTACC TTGCTTAGAAAAGTTATGCT |
baeR-dw-Sac I_R | AAATGAGCTC ATGCTTTAGGGGTGGCTTCT |
baeR-up-check_R | CTTTCCCAGTGGTGGTTACG |
baeR-dw-check_R | GACGGACGTGGCTTACTCAT |
pWH1266 check_F | TGCCACCTGACGTCTAAGAA |
pWH1266 check_R | TCATACACGGTGCCTGACTG |
sacB _F | AGTTTTGTTCAGCGGCTTGT |
sacB _R | GGTCAGGTTCAGCCACATTT |
baeR _F | GGATGGTTTAACGATTTGCC |
baeR _R | TCCACTCATGACGGTTACCA |
baeS _F | CTTTCCCAGTGGTGGTTACG |
baeS _R | GAGCCAAGTCTGCCAAATCT |
baeS probe_F | CCTCAATACTGGTGAAACCA |
baeS probe_R | TCCCCCAATCATGATAAACG |
rpoB _F | AGTCACGCGAAGTTGAAGGT |
rpoB _R | GCGGTATGGAGTTTCCAAGA |
16 s rRNA_F | GTAGCTTGCTACTGGACCTAG |
16 s rRNA_R | CATACTCTAGCTCACCAGTATCG |
Kan-2r-EcoR I_F | AATAGAATTC ACATCTCAACCATCATCG |
Kan-2r-EcoR I_R | AATAGAATTC CATCTCAACCCTGAAGC |
baeR-Xho I_F | TTAACTCGAG CATGTTTCATGATGGTC |
baeR-Xba I_R | TAGCTCTAGA TTATTCTTCTGGATATTCG |
qbaeS _F | GCCATTCAGCAGCATTCTTTC |
qbaeS _R | ATTTACCGTTCCCGCATCTG |
qbaeR _F | TGACAGCACGTACCGAAGAAA |
qbaeR _R | CATAATCATCTGCCCCCATGT |
qadeB _F | ACAAGACCGCGCTAACTTAGGT |
qadeB _R | TGCCATTGCCATAAGTTCATCT |
qadeA1 _F | CCTCAAGCGCTATTGGTTCCT |
qadeA1 _R | CCTGAGGCTCGCCACTGA |
qadeA2 _F | TTTGAGGCCGATGTAAATAGCA |
qadeA2 _R | GTCTTGCCACCTCAGCTTCAG |
q16s rRNA_F | AGCATTTCGGATGGGAACTTTA |
q16s rRNA_R | GTCGTCCCCGCCTTCCT |