Figure 2From: Role of the BaeSR two-component system in the regulation of Acinetobacter baumannii adeAB genes and its correlation with tigecycline susceptibility Evaluation of baeR and baeS expression. (A) The co-transcription of baeR and baeS was determined by agarose gel electrophoresis of the product obtained by reverse transcription polymerase chain reaction (RT-PCR). Lane 2 (cDNA) and 3 (genomic DNA) reveal a 793-bp DNA fragment covering the junction between both the baeR and baeS genes. (B) The relative transcript levels of baeR and baeS, as determined by RT-PCR, under different osmolarity conditions. The cells were grown on Luria-Bertani (LB) agar with or without 20% sucrose (37°C, 220 rpm). 16S rRNA and rpoB genes were used as controls. The expression levels of baeR and baeS were 2.3- and 6.7-fold higher in cells experiencing osmotic stress than those in cells grown without sucrose. The results are displayed as the means ± SD from four independent experiments. *, P < 0.05; **, P < 0.01.Back to article page