Type three secretion system-mediated escape of Burkholderia pseudomallei into the host cytosol is critical for the activation of NFκB

Table 2 Plasmids used for cellular transfection in the study

Plasmid	Relevant characteristic(s)^a	Source or reference
pK18mobsacB	Conjugative, suicide vector containing sacB gene, Km^r	[42]
pUC18T-mini-Tn7T-Zeo-lox	Source of zeocin resistance gene, Ap^r, Zeo^r	[43]
pNFκB-SEAP	Reporter vector containing NFκB enhancer element fused to SEAP gene, Amp^R	BD Clontech
pcDNA3.1/V5-His TOPO	Expression Vector, Amp^R	Life Technologies
pCMV-FLAG-MAT-Tag-1	Expression Vector, Amp^R	Sigma
pcDNA-bopA	bopA gene cloned into pcDNA3.1/V5-His TOPO by TA-cloning, Amp^R	This study
pCMV-bopC	bopC gene cloned into pCMV-FLAG-MAT-Tag-1, Amp^R	This study
pRK5myc-BopE	bopE gene cloned into pRK5myc, Amp^R	[23]
pRK5myc-SopE	sopE gene cloned into pRK5myc, Amp^R	[23]

ISSN: 1471-2180