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Figure 2 | BMC Microbiology

Figure 2

From: Type three secretion system-mediated escape of Burkholderia pseudomallei into the host cytosol is critical for the activation of NFκB

Figure 2

Deletion of T3SS3 effector genes had little effect on TLR independent NFκB activation by B. pseudomallei . A) HEK293T cells were transfected with pNFκB-SEAP for 24 hr. The transfected cells were infected with wildtype KHW and mutants at MOI of 10:1 for 6 hr. Supernatants were collected for SEAP assay. B) HEK293T cells were infected with respective strains for 6 hr. Cells were lysed and plated for intracellular bacterial count. C) HEK293T cells were infected with respective strains for 12 hr. The infected cells were fixed, stained with Giemsa and visualized under 10x magnification on a light microscope. Asterisks * and ** indicate significant differences of p < 0.05 and p < 0.01 between B. pseudomallei wildtype and mutant strains respectively.

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