Figure 1From: Type three secretion system-mediated escape of Burkholderia pseudomallei into the host cytosol is critical for the activation of NFκB TLR independent NFκB activation by B. pseudomallei requires T3SS3. A) HEK293T cells were transfected with pNFκB-SEAP for 24 hr. The transfected cells were infected with wildtype KHW and mutants at MOI of 10:1 for 6 hr. Supernatants were collected for SEAP assay. B) HEK293T cells were infected with respective strains for 6 hr. Cells were lysed and plated for intracellular bacterial count. C) HEK293T cells were transfected with pNFκB-SEAP for 24 hr. The transfected cells were infected with wildtype KHW and mutants at indicated MOI for 6 hr. Supernatants were collected for SEAP assay. D) HEK293T cells were infected with respective strains for 6 hr. Supernatants were collected for lactate dehydrogenase (LDH) assay. Asterisks * and ** indicate significant differences of p < 0.05 and p < 0.01 between B. pseudomallei wildtype and mutant strains respectively.Back to article page