Table 4 Oligonucleotide primers used in this study
Primer name | Sequence |
|---|---|
Cloning | Â |
AttbPc12g07500-RV | GGGGACAAGTTTGTACAAAAAAGCAGGCTATGATTTGGAAATCTCTCTTTAGTGCTTTGGCC |
AttbPc12g07500-RV | GGGGACCACTTTGTACAAGAAAGCTGGGTTCACATGCTACCTCTAGCC |
AttbMF(Alpha)1-FW | GGGGACAAGTTTGTACAAAAAAGCAGGCTATGAGATTTCCTTCAATTTTTACTGC |
MF(Alpha)1 Spe 1-RV | GGCTAGCACTAGTGTAAGCTTCAGCCTCTCTTTTCTCG |
Pc12g07500 Spe 1-FW | CGTTAGACTAGTGCCCCAGCTCTCGATTCAGAGC |
Expression (RT-qPCR) | Â |
Pc12g07500 208 FW | TCCGGGTACCTTGACTGTGC |
Pc12g07500 208 RW | CCCGAGCCACCTTGAAAGAC |
Pc12g07500 209 FW | TCCGGGTACCTTGACTGTGC |
Pc12g07500 209 RV | CCCGAGCCACCTTGAAAGAC |
Pc12g13330 210 FW | GACGCTTTGCAACCAGAAGG |
Pc12g13330 210 RV | TGGGACGGTACCCAAAGATG |
3′ FW ACT1 | GGCTTCTTTGACTACCTTCCA |
3′ RV ACT1 | AGAAACACTTGTGGTGAACGA |