Functional characterization of a Penicillium chrysogenum mutanase gene induced upon co-cultivation with Bacillus subtilis

Table 2 Transcript levels of heterologously expressed putative mutanase genes from P. chrysogenum in S. cerevisiae

Gene and S. cerevisiae strain/relevant genotype	Gene expression relative to reference gene ACT1
Pc12g07500 IME208 [pUDE 230 (TDH3 _p ::Pc12g07500:: CYC1_t)]	1.52 ± 0.16
Pc12g07500 IME209 [pUDE 249 (TDH3 _p ::MF(Alpha)1 ^1–270 -Pc12g07500-∆^1–63 ::CYC1 _t)]	1.49 ± 0.33
Pc12g13330 IME210 [pUDE 250 (URA3 TDH3 _p ::MF(Alpha)1 ^1–270 -Pc12g13330::CYC1 _t)]	1.13 ± 0.08

Expression levels were estimated by RT-qPCR using ACT1. Data are average ± mean deviation of duplicate analyses.

ISSN: 1471-2180