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Table 1 Addition of PBS to obtain stools with a range of water content

From: Processing faecal samples: a step forward for standards in microbial community analysis

ID

Weight (mg)

Presence of beads

PBS (μl)

Water content

L#

250

yes

-

75.0%

DL#.00

250

yes

0

75.0%

DL#.25

187.5

yes

62.5

80.0%

DL#.50

125

yes

125.0

87.5%

DL#.75

62.5

yes

187.5

93.8%

DL#.90

25

yes

225.0

97.5%

DL#.98

5

yes

245.0

99.5%

DL#B.00

250

yes

-

75.0%

DL#B.25

187.5

yes

-

75.0%

DL#B.50

125

yes

-

75.0%

DL#B.75

62.5

yes

-

75.0%

DL#B.90

25

yes

-

75.0%

DL#B.98

5

yes

-

75.0%

DL#P.50

125

-

125.0

87.5%

DL#P.75

62.5

-

187.5

93.8%

DL#P.90

25

-

225.0

97.5%

DL#P.98

5

-

245.0

99.5%

DL#C.50

125

-

-

75.0%

DL#C.75

62.5

-

-

75.0%

DL#C.90

25

-

-

75.0%

DL#C.98

5

-

-

75.0%

  1. # indicates the identification number for each subject.
  2. L# = stands for layer in the homogenisation study.
  3. DL# = the “D” stands for diarrhoea in the water content study; the “L” refers to samples that have been also used in the homogenisation study, that contained PBS and underwent a bead-beating step.
  4. DL#B = samples that did not contain PBS but underwent a bead-beating step.
  5. DL#P = samples that contained PBS but did not undergo a bead-beating step.
  6. DL#C = samples that did not contain PBS and did not undergo a bead-beating step.