Figure 4
From: Delineation of immunodominant and cytadherence segment(s) of Mycoplasma pneumoniae P1 gene
Recombinant P1 protein fragments are recognized by anti- M. pneumoniae antibody and by sera of M. pneumoniae infected patients. (A) (I) Coomassie blue stained SDS-PAGE analysis of purified M. pneumoniae P1 protein fragments; rP1-I, rP1-II, rP1-III and rP1-IV. Immuno blot analysis of purified P1 protein fragments; rP1-I, rP1-II, rP1-III and rP1-IV using anti-M. pneumoniae antibody (II) and using pooled sera of M. pneumoniae infected patients (III). (B) Immuno blot analysis of purified M. pneumoniae P1 protein fragments rP1-I, rP1-II, rP1-III and rP1-IV with several sera of M. pneumoniae infected patients. PM: Prestained protein marker; PC: positive control; NC: Negative control; Numbers over the blot indicate serial number of sera of M. pneumoniae infected patients tested for these experiments.