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Table 4 Species assignation of Lactobacillus isolates based on MALDI-TOF analyses, 16S RNA and rpoA genes sequencing

From: Screening of Lactobacillus spp. for the prevention of Pseudomonas aeruginosa pulmonary infections

Strain Mass spectrometry 16S RNA gene sequencing rpoA gene sequencing API 50 CHL
Identification Score* Identification NCBI accession number (GenBank) Identification NCBI accession number (GenBank) Identification Score
ES.A.2 L. fermentum 1.916 L. fermentum [KC762296] L. fermentum [KC861367] L. fermentum 96.3%
ES.D.88 L. paracasei 2.458 L. casei group [KC762297] L. paracasei [KC861369] L. paracasei 98.4%
ES.F.115 L. fermentum 2.21 L. fermentum [KC762298] L. fermentum [KC861368] L. fermentum 98.5%
Od.76*** L. zeae 2.089 L. casei group [KC762299] L. zeae/casei [KC861370] - ** - **
L. casei 2.043
K.C6.3.1D L. fermentum 2.18 L. fermentum [KC762300] L. fermentum [KC861371] L. fermentum 99.7%
K.C6.3.1E L. fermentum 2.109 L. fermentum [KC762301] L. fermentum [KC861372] L. fermentum 99.7%
K.V9.3.2B L. fermentum 2.163 L. fermentum [KC762302] L. fermentum [KC861373] L. fermentum 99.7%
K.V9.3.2C L. fermentum 2.223 L. fermentum [KC762303] L. fermentum [KC861374] L. fermentum 99.8%
  1. *An isolate was considered well identified at the species level with a MALDI-TOF mass spectrometry score ≥ 1.9. ** The API 50 CHL gallery is not designed to identify L. zeae. ***Od.76 was formally identified as a L. zeae by sequencing the pheS gene (Genbank accession number: KJ402364).