Discovery of a novel rumen methanogen in the anaerobic fungal culture and its distribution in the rumen as revealed by real-time PCR

Table 2 The 16S rRNA copy numbers of the total Archaea and the novel RCC species in the rumen as quantified by real-time PCR

Level of concentrate inclusion*	Archaea			The novel RCC species			The novel RCC species/Archaea
	Epithelium × 10⁶ cm²	Liquid × 10⁶ ml	Solid × 10⁶ g	Epithelium × 10⁶ cm²	Liquid × 10⁶ ml	Solid × 10⁶ g	Epithelium %	Liquid %	Solid %
High (65%)	33.25	133.94	2079.26	0.50^a	14.44	50.30	1.56	12.01	2.85
Medium (40%)	36.68	248.93	1857.66	0.66^a	30.97	38.46	12.90	19.06	5.71
Low (0%)	16.34	162.69	1385.19	2.56^b	93.45	76.04	29.10	56.47	2.68
SEM	6.22	35.73	285.15	0.40	16.56	10.73	7.98	9.23	0.78
P-value	0.413	0.450	0.661	0.034	0.106	0.393	0.421	0.086	0.219

^{a, b, c}, means with different letters in the same column are different P < 0.05; n = 3.
^*, The pH value of rumen content, 5.60 ± 0.11 (High); 5.79 ± 0.15 (Medium); 6.17 ± 0.25 (Low).

ISSN: 1471-2180