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Table 2 The 16S rRNA copy numbers of the total Archaea and the novel RCC species in the rumen as quantified by real-time PCR

From: Discovery of a novel rumen methanogen in the anaerobic fungal culture and its distribution in the rumen as revealed by real-time PCR

Level of concentrate inclusion* Archaea The novel RCC species The novel RCC species/Archaea
  Epithelium × 106 cm2 Liquid × 106 ml Solid × 106 g Epithelium × 106 cm2 Liquid × 106 ml Solid × 106 g Epithelium % Liquid % Solid %
High (65%) 33.25 133.94 2079.26 0.50a 14.44 50.30 1.56 12.01 2.85
Medium (40%) 36.68 248.93 1857.66 0.66a 30.97 38.46 12.90 19.06 5.71
Low (0%) 16.34 162.69 1385.19 2.56b 93.45 76.04 29.10 56.47 2.68
SEM 6.22 35.73 285.15 0.40 16.56 10.73 7.98 9.23 0.78
P-value 0.413 0.450 0.661 0.034 0.106 0.393 0.421 0.086 0.219
  1. a, b, c, means with different letters in the same column are different P < 0.05; n = 3.
  2. *, The pH value of rumen content, 5.60 ± 0.11 (High); 5.79 ± 0.15 (Medium); 6.17 ± 0.25 (Low).